BMRB Entry 5490

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5490

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Title:
Sequence-specific 1H, 13C, 15N Resonance Assigments of the Major Cherry Allergen Pru av 1 Mutant E45W
Deposition date:
2002-08-05
Original release date:
2003-10-06
Authors:
Neudecker, Philipp; Lehmann, Katrin; Nerkamp, Joerg; Schweimer, Kristian; Sticht, Heirich; Boehm, Markus; Scheurer, Stephan; Vieths, Stefan; Roesch, Paul
Citation:

Citation: Neudecker, Philipp; Lehmann, Katrin; Nerkamp, Joerg; Haase, Tanja; Wangorsch, Andrea; Foetisch, Kay; Hoffmann, Silke; Roesch, Paul; Vieths, Stefan; Scheurer, Stephan. "Mutational epitope analysis of Pru av 1 and Api g 1, the major allergens of cherry (Prunus avium) and celery (Apium graveolens): correlating IgE reactivity with three-dimensional structure"  Biochem. J. 376, 97-107 (2003).
PubMed: 12943529

Assembly members:

Assembly members:
Pru av 1, polymer, 159 residues, 17586 Da.

Natural source:

Natural source:   Common Name: sweet cherry   Taxonomy ID: 42229   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Prunus avium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pru av 1: GVFTYESEFTSEIPPPRLFK AFVLDADNLVPKIAPQAIKH SEILWGDGGPGTIKKITFGE GSQYGYVKHKIDSIDKENYS YSYTLIEGDALGDTLEKISY ETKLVASPSGGSIIKSTSHY HTKGNVEIKEEHVKAGKEKA SNLFKLIETYLKGHPDAYN

Data sets:
Data typeCount
1H chemical shifts1017
13C chemical shifts558
15N chemical shifts156
coupling constants96

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Pru av 1 E45W1

Entities:

Entity 1, Pru av 1 E45W 159 residues - 17586 Da.

1   GLYVALPHETHRTYRGLUSERGLUPHETHR
2   SERGLUILEPROPROPROARGLEUPHELYS
3   ALAPHEVALLEUASPALAASPASNLEUVAL
4   PROLYSILEALAPROGLNALAILELYSHIS
5   SERGLUILELEUTRPGLYASPGLYGLYPRO
6   GLYTHRILELYSLYSILETHRPHEGLYGLU
7   GLYSERGLNTYRGLYTYRVALLYSHISLYS
8   ILEASPSERILEASPLYSGLUASNTYRSER
9   TYRSERTYRTHRLEUILEGLUGLYASPALA
10   LEUGLYASPTHRLEUGLULYSILESERTYR
11   GLUTHRLYSLEUVALALASERPROSERGLY
12   GLYSERILEILELYSSERTHRSERHISTYR
13   HISTHRLYSGLYASNVALGLUILELYSGLU
14   GLUHISVALLYSALAGLYLYSGLULYSALA
15   SERASNLEUPHELYSLEUILEGLUTHRTYR
16   LEULYSGLYHISPROASPALATYRASN

Related Database Links:

BMRB 4671
PDB 1E09 1H2O
EMBL CAM35495
GB AAC02632 ABB78006 ABW99628 ACE80940 EMJ24835
REF XP_007223636
SP O24248
AlphaFold O24248

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SPARKY: Backbone or all simulated peaks