BMRB Entry 5372

Title:
Solution Structure Of Reduced Horse Heart Cytochrome c in 30% Acetonitrile Solution
Deposition date:
2002-05-09
Original release date:
2003-06-09
Authors:
Sivakolundu, Sivashankar; Mabrouk, Patricia
Citation:

Citation: Sivakolundu, Sivashankar; Mabrouk, Patricia. "Structure-function Relationship of Reduced Cytochrome c Probed by Complete Solution Structure Determination in 30% Acetonitrile/Water Solution "  J. Biol. Inorg. Chem. 8, 527-539 (2003).
PubMed: 12764601

Assembly members:

Assembly members:
cytochrome c, polymer, 104 residues, 12384 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Horse   Taxonomy ID: 9796   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Equus caballus

Experimental source:

Experimental source:   Production method: vendor

Data sets:
Data typeCount
1H chemical shifts677
coupling constants73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYTOCHROME C1
2HEME C2

Entities:

Entity 1, CYTOCHROME C 104 residues - 12384 Da.

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   VALGLNLYSCYSALAGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYPHETHRTYRTHRASP
6   ALAASNLYSASNLYSGLYILETHRTRPLYS
7   GLUGLUTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEALAGLYILELYSLYSLYSTHRGLU
10   ARGGLUASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, HEME C - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c 5 mM; PROTOPORPHYRIN IX CONTAINING FE 5 mM; phosphate buffer 50 mM; acetonitrile 30 % v/v; H2O 70 % v/v

sample_cond_1: pH: 7.2; temperature: 298 K; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1

Software:

RNMR v1.0 - collection

FELIX v2000 - processing

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • home built home built 591.1 MHz

Related Database Links:

SWISS-PROT P68097 P68096 P00004
REF XP_001498872
PRF 610169A
GenBank AAB33495
PDB
BMRB 948 947 946 944 673 672 665 645 630 5830 5829 5828 5827 5660 546 545 544 543 5026 499 4810 4809 4808 4805 439 438 437 436 4189 336 317 316 286 285 274 244 243 2368 2367 2366 224 220 216 1789 1787 1785 1783 1736 1404 1171 1170 1116 1114 1113 1112 1111 1110 1109 1108 1107 1058