BMRB Entry 5344

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5344

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Title:
1H, 15N Assignment of Neocarzinostatin Apo-Protein Bound to a Synthetic Chromophore
Deposition date:
2002-04-12
Original release date:
2006-04-05
Authors:
Urbaniak, Michael; Muskett, Frederick; Finucane, Michael; Caddick, Stephen; Woolfson, Derek
Citation:

Citation: Urbaniak, Michael; Muskett, Frederick; Finucane, M.; Caddick, Stephen; Woolfson, Derek. "Solution Structure of a Novel Chromoprotein dervived from apo-Neocarzinostatin and a Synthetic Chromophore "  Biochemistry 41, 11731-11739 (2002).
PubMed: 12269815

Assembly members:

Assembly members:
Neocarzinostatin apo-protein, polymer, 122 residues, 12213 Da.
SM, non-polymer, 150.360 Da.

Natural source:

Natural source:   Common Name: Streptomyces carzinostaticus   Taxonomy ID: 1897   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces carzinostaticus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pCANTABB

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neocarzinostatin apo-protein: HHHHHHLQGAAPTATVTPSS GLSDGTVVKVAGAGLQAGTA YDVGQCAWVDTGVLACNPAD FSSVTADANGSASTSLTVRR SFEGFLFDGTRWGTVDCTTA ACQVGLSDAAGNGPEGVAIS FN

Data sets:
Data typeCount
15N chemical shifts113
1H chemical shifts552

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Neocarzinostatin apo-protein1
2Napthoate ester2

Entities:

Entity 1, Neocarzinostatin apo-protein 122 residues - 12213 Da.

1   HISHISHISHISHISHISLEUGLNGLYALA
2   ALAPROTHRALATHRVALTHRPROSERSER
3   GLYLEUSERASPGLYTHRVALVALLYSVAL
4   ALAGLYALAGLYLEUGLNALAGLYTHRALA
5   TYRASPVALGLYGLNCYSALATRPVALASP
6   THRGLYVALLEUALACYSASNPROALAASP
7   PHESERSERVALTHRALAASPALAASNGLY
8   SERALASERTHRSERLEUTHRVALARGARG
9   SERPHEGLUGLYPHELEUPHEASPGLYTHR
10   ARGTRPGLYTHRVALASPCYSTHRTHRALA
11   ALACYSGLNVALGLYLEUSERASPALAALA
12   GLYASNGLYPROGLUGLYVALALAILESER
13   PHEASN

Entity 2, Napthoate ester - Sm - 150.360 Da.

1   SM

Related Database Links:

BMRB 1766 5343 5969 6888 695
PDB 1J5H 1J5I 1NCO 1NOA 1O5P 2G0K 2G0L
DBJ BAA01764 BAB83894
GB AAB28103 AAM77994
SP P0A3R9 P0A3S0
AlphaFold P0A3R9 P0A3S0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks