BMRB Entry 5058
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5058
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Stangler, Thomas; Mayr, Lorenz; Dingley, Andrew; Luge, Cornelia; Willbold, Dieter. "Letter to the Editor: Sequence specific 1H, 13C and 15N resonance assignments of
human GABA receptor associated protein" J. Biomol. NMR 21, 183-184 (2001).
Assembly members:
GABARAP, polymer, 119 residues, 14077 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Vector: pGEX-4T-1
Entity Sequences (FASTA):
GABARAP: GSMKFVYKEEHPFEKRRSEG
EKIRKKYPDRVPVIVEKAPK
ARIGDLDKKKYLVPSDLTVG
QFYFLIRKRIHLRAEDALFF
FVNNVIPPTSATMGQLYQEH
HEEDFFLYIAYSDESVYGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 844 |
| 13C chemical shifts | 527 |
| 15N chemical shifts | 105 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABARAP | 1 |
Entities:
Entity 1, GABARAP 119 residues - 14077 Da.
| 1 | GLY | SER | MET | LYS | PHE | VAL | TYR | LYS | GLU | GLU | ||||
| 2 | HIS | PRO | PHE | GLU | LYS | ARG | ARG | SER | GLU | GLY | ||||
| 3 | GLU | LYS | ILE | ARG | LYS | LYS | TYR | PRO | ASP | ARG | ||||
| 4 | VAL | PRO | VAL | ILE | VAL | GLU | LYS | ALA | PRO | LYS | ||||
| 5 | ALA | ARG | ILE | GLY | ASP | LEU | ASP | LYS | LYS | LYS | ||||
| 6 | TYR | LEU | VAL | PRO | SER | ASP | LEU | THR | VAL | GLY | ||||
| 7 | GLN | PHE | TYR | PHE | LEU | ILE | ARG | LYS | ARG | ILE | ||||
| 8 | HIS | LEU | ARG | ALA | GLU | ASP | ALA | LEU | PHE | PHE | ||||
| 9 | PHE | VAL | ASN | ASN | VAL | ILE | PRO | PRO | THR | SER | ||||
| 10 | ALA | THR | MET | GLY | GLN | LEU | TYR | GLN | GLU | HIS | ||||
| 11 | HIS | GLU | GLU | ASP | PHE | PHE | LEU | TYR | ILE | ALA | ||||
| 12 | TYR | SER | ASP | GLU | SER | VAL | TYR | GLY | LEU |
Samples:
sample_1: GABARAP, [U-15N; U-13C], 0.8 mM; sodium chloride 100 mM; potassium chloride 100 mM; sodium oxalate 25 mM; sodium azide 0.02%; EDTA 0.05 mM; PMSF 0.1 mM; Glycerol mM
conditions_1: pH: 5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HCCONH-TOCSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
Software:
NMRPipe - data processing
NMRView v5.0.3 - spectral analysis
NMR spectrometers:
- Varian Unity INOVA 750 MHz
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 5064 5128 |
| PDB | |
| DBJ | BAB21549 BAB22426 BAB27806 BAG35138 |
| EMBL | CAG00707 CAG33324 CAG47031 CAP17839 CAP17840 |
| GB | AAD02337 AAD32455 AAD47641 AAD47642 AAD47643 |
| REF | NP_001011192 NP_001013278 NP_001029220 NP_001075611 NP_001084530 |
| SP | O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7 |
| TPG | DAA18837 |
| AlphaFold | O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks