BMRB Entry 5128
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5128
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Kouno, Takahide; Miura, Kazunori; Kanematsu, Takashi; Shirakawa, Masahiro; Hirata, Masato; Kawano, Keiichi. "1H, 13C and 15N Resonance Assignments of GABARAP, GABAA Receptor Associated
Protein" J. Biomol. NMR 22, 97-98 (2002).
PubMed: 11885988
Assembly members:
GABAA receptor associated protein, polymer, 117 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
GABAA receptor associated protein: MKFVYKEEHPFEKRRSEGEK
IRKKYPDRVPVIVEKAPKAR
IGDLDKKKYLVPSDLTVGQF
YFLIRKRIHLRAEDALFFFV
NNVIPPTSATMGQLYQEHHE
EDFFLYIAYSDESVYGL
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 654 |
| 13C chemical shifts | 199 |
| 15N chemical shifts | 97 |
| coupling constants | 59 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABARAP | 1 |
Entities:
Entity 1, GABARAP 117 residues - Formula weight is not available
| 1 | MET | LYS | PHE | VAL | TYR | LYS | GLU | GLU | HIS | PRO | ||||
| 2 | PHE | GLU | LYS | ARG | ARG | SER | GLU | GLY | GLU | LYS | ||||
| 3 | ILE | ARG | LYS | LYS | TYR | PRO | ASP | ARG | VAL | PRO | ||||
| 4 | VAL | ILE | VAL | GLU | LYS | ALA | PRO | LYS | ALA | ARG | ||||
| 5 | ILE | GLY | ASP | LEU | ASP | LYS | LYS | LYS | TYR | LEU | ||||
| 6 | VAL | PRO | SER | ASP | LEU | THR | VAL | GLY | GLN | PHE | ||||
| 7 | TYR | PHE | LEU | ILE | ARG | LYS | ARG | ILE | HIS | LEU | ||||
| 8 | ARG | ALA | GLU | ASP | ALA | LEU | PHE | PHE | PHE | VAL | ||||
| 9 | ASN | ASN | VAL | ILE | PRO | PRO | THR | SER | ALA | THR | ||||
| 10 | MET | GLY | GLN | LEU | TYR | GLN | GLU | HIS | HIS | GLU | ||||
| 11 | GLU | ASP | PHE | PHE | LEU | TYR | ILE | ALA | TYR | SER | ||||
| 12 | ASP | GLU | SER | VAL | TYR | GLY | LEU |
Samples:
sample_1: GABAA receptor associated protein 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%
sample_2: GABAA receptor associated protein, [U-15N], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%
sample_3: GABAA receptor associated protein, [U-13C; U-15N], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%
Ex-cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 1H-1H-15N TOCSY | not available | not available | not available |
| 1H-13C-1H HCCH-TOCSY | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HNHA | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 5058 5064 |
| PDB | |
| DBJ | BAB21549 BAB22426 BAB27806 BAG35138 |
| EMBL | CAG00707 CAG33324 CAG47031 CAP17839 CAP17840 |
| GB | AAD02337 AAD32455 AAD47641 AAD47642 AAD47643 |
| REF | NP_001011192 NP_001013278 NP_001029220 NP_001075611 NP_001084530 |
| SP | O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7 |
| TPG | DAA18837 |
| AlphaFold | O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks