BMRB Entry 5128

Title:
GABARAP, GABAA receptor associated protein
Deposition date:
2001-08-31
Original release date:
2002-04-08
Authors:
Kouno, Takahide; Miura, Kazunori; Kanematsu, Takashi; Shirakawa, Masahiro; Hirata, Masato; Kawano, Keiichi
Citation:

Citation: Kouno, Takahide; Miura, Kazunori; Kanematsu, Takashi; Shirakawa, Masahiro; Hirata, Masato; Kawano, Keiichi. "1H, 13C and 15N Resonance Assignments of GABARAP, GABAA Receptor Associated Protein"  J. Biomol. NMR 22, 97-98 (2002).
PubMed: 11885988

Assembly members:

Assembly members:
GABAA receptor associated protein, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts654
13C chemical shifts199
15N chemical shifts97
coupling constants59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GABARAP1

Entities:

Entity 1, GABARAP 117 residues - Formula weight is not available

1   METLYSPHEVALTYRLYSGLUGLUHISPRO
2   PHEGLULYSARGARGSERGLUGLYGLULYS
3   ILEARGLYSLYSTYRPROASPARGVALPRO
4   VALILEVALGLULYSALAPROLYSALAARG
5   ILEGLYASPLEUASPLYSLYSLYSTYRLEU
6   VALPROSERASPLEUTHRVALGLYGLNPHE
7   TYRPHELEUILEARGLYSARGILEHISLEU
8   ARGALAGLUASPALALEUPHEPHEPHEVAL
9   ASNASNVALILEPROPROTHRSERALATHR
10   METGLYGLNLEUTYRGLNGLUHISHISGLU
11   GLUASPPHEPHELEUTYRILEALATYRSER
12   ASPGLUSERVALTYRGLYLEU

Samples:

sample_1: GABAA receptor associated protein 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%

sample_2: GABAA receptor associated protein, [U-15N], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%

sample_3: GABAA receptor associated protein, [U-13C; U-15N], 1 mM; sodium phosphate 20 mM; NaCl 50 mM; NaN3 0.02 mM; H2O0 – 90%; D2O10 – 100%

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
2D 1H-15N HSQCnot availablenot availablenot available
3D 1H-1H-15N NOESYnot availablenot availablenot available
3D 1H-1H-15N TOCSYnot availablenot availablenot available
1H-13C-1H HCCH-TOCSYnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D HNCAnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HNHAnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 5058 5064
PDB
DBJ BAB21549 BAB22426 BAB27806 BAG35138
EMBL CAG00707 CAG33324 CAG47031 CAP17839 CAP17840
GB AAD02337 AAD32455 AAD47641 AAD47642 AAD47643
REF NP_001011192 NP_001013278 NP_001029220 NP_001075611 NP_001084530
SP O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7
TPG DAA18837
AlphaFold Q9GJW7 O95166 P60517 Q8MK68 Q9DCD6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks