BMRB Entry 4813

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4813

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Title:
NMR structure of lac repressor HP62-DNA complex
Deposition date:
2000-08-22
Original release date:
2000-11-13
Authors:
Spronk, C.; Bonvin, A.; Radha, P.; Melacini, G.; Boelens, R.; Kaptein, R.
Citation:

Citation: Spronk, C.; Bonvin, A.; Radha, P.; Melacini, G.; Boelens, R.; Kaptein, R.. "The Solution Structure of Lac Repressor Headpiece 62 Complexed to a Symmetrical Lac Operator"  Structure 7, 1483-1492 (1999).

Assembly members:

Assembly members:
LAC REPRESSOR, polymer, 62 residues, Formula weight is not available
LAC OPERATOR, polymer, 22 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PGP1-2, PET-HP62

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LAC REPRESSOR: MKPVTLYDVAEYAGVSYQTV SRVVNQASHVSAKTREKVEA AMAELNYIPNRVAQQLAGKQ SL
LAC OPERATOR: GAATTGTGAGCGCTCACAAT TC

Data sets:
Data typeCount
1H chemical shifts595
13C chemical shifts169
15N chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LAC REPRESSOR HP62 A1
2LAC REPRESSOR HP62 B1
3LAC OPERATOR C2
4LAC OPERATOR D2

Entities:

Entity 1, LAC REPRESSOR HP62 A 62 residues - Formula weight is not available

1   METLYSPROVALTHRLEUTYRASPVALALA
2   GLUTYRALAGLYVALSERTYRGLNTHRVAL
3   SERARGVALVALASNGLNALASERHISVAL
4   SERALALYSTHRARGGLULYSVALGLUALA
5   ALAMETALAGLULEUASNTYRILEPROASN
6   ARGVALALAGLNGLNLEUALAGLYLYSGLN
7   SERLEU

Entity 2, LAC OPERATOR C 22 residues - Formula weight is not available

1   DGDADADTDTDGDTDGDADG
2   DCDGDCDTDCDADCDADADT
3   DTDC

Related Database Links:

BMRB 1066 127 1494 1552 2956 32 5345 5791 661 736 848 849 96
PDB 1CJG 1EFA 1JWL 1JYE 1JYF 1L1M 1LBG 1LBH 1LBI 1LCC 1LCD 1LQC 1OSL 2KEI 2KEJ 2KEK 2PE5 3EDC
DBJ BAB20667 BAB33821 BAD00175 BAD20286 BAD20288
EMBL CAA07594 CAA07597 CAA11118 CAA11119 CAA11120
GB AAA24052 AAA56744 AAA56768 AAA57088 AAA57091
REF NP_286086 NP_308425 NP_414879 WP_000248559 WP_000248563
SP P03023
AlphaFold P03023

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SPARKY: Backbone or all simulated peaks