BMRB Entry 5345

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PDB ID: 1l1m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5345
MolProbity Validation Chart

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Title:
Assignment of lac repressor headpiece complexed of its natural operator
Deposition date:
2002-04-19
Original release date:
2002-06-07
Authors:
Kalodimos, C.; Bonvin, A.; Salinas, R.; Wechselberger, R.; Boelens, R.; Kaptein, R.
Citation:

Citation: Kalodimos, Charalampos; Bonvin, A.; Salinas, R.; Wechselberger, R.; Boelens, R.; Kaptein, R.. "PLASTICITY IN PROTEIN-DNA RECOGNITION: LAC REPRESSOR INTERACTS WITH ITS NATURAL OPERATOR THROUGH ALTERNATIVE CONFORMATIONS OF ITS DNA-BINDING DOMAIN"  EMBO J. 21, 2866-2876 (2002).
PubMed: 12065400

Assembly members:

Assembly members:
lactose repressor-operator, polymer, 62 residues, 7000 Da.
lactose repressor-operator DNA, polymer, 23 residues, Formula weight is not available
lactose repressor-operator DNA, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lactose repressor-operator: MKPVTLYDVAEYAGVSYQTV SRVVNQASHVSAKTREKVEA AMAELNYIPNRCAQQLAGKQ SL
lactose repressor-operator DNA: GAATTGTGAGCGGATAACAA TTT
lactose repressor-operator DNA: AAATTGTTATCCGCTCACAA TTC

Data typeCount
13C chemical shifts458
1H chemical shifts1207
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Lactose operon repressor, chain A1
2Lactose operon repressor, chain B1
3LAC-DNA, chain A2
4LAC-DNA, chain B3

Entities:

Entity 1, Lactose operon repressor, chain A 62 residues - 7000 Da.

1   METLYSPROVALTHRLEUTYRASPVALALA
2   GLUTYRALAGLYVALSERTYRGLNTHRVAL
3   SERARGVALVALASNGLNALASERHISVAL
4   SERALALYSTHRARGGLULYSVALGLUALA
5   ALAMETALAGLULEUASNTYRILEPROASN
6   ARGCYSALAGLNGLNLEUALAGLYLYSGLN
7   SERLEU

Entity 2, LAC-DNA, chain A 23 residues - Formula weight is not available

1   DGDADADTDTDGDTDGDADG
2   DCDGDGDADTDADADCDADA
3   DTDTDT

Entity 3, LAC-DNA, chain B 23 residues - Formula weight is not available

1   DADADADTDTDGDTDTDADT
2   DCDCDGDCDTDCDADCDADA
3   DTDTDC

Related Database Links:

BMRB 1066 127 1494 1552 32 4813 5791 661 736 848 849 96
PDB 1CJG 1EFA 1JWL 1JYE 1JYF 1L1M 1LBG 1LBH 1LBI 1LCC 1LCD 1LQC 1OSL 2KEI 2KEJ 2KEK 3EDC
DBJ BAB20667 BAB33821 BAD00175 BAD20286 BAD20288
EMBL CAA07594 CAA07597 CAA11118 CAA11119 CAA11120
GB AAA24052 AAA56744 AAA56768 AAA57088 AAA57091
REF NP_286086 NP_414879 WP_000718174 WP_000805876 WP_000805884
SP P03023
AlphaFold P03023

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