BMRB Entry 4700

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PDB ID: 1cf4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4700
MolProbity Validation Chart

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Title:
Structure of Cdc42 bound to the GTPase Binding Domain of PAK
Deposition date:
2000-03-23
Original release date:
2000-12-18
Authors:
Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest
Citation:

Citation: Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest. "Structure of Cdc42 bound to the GTPase Binding Domain of PAK"  Nat. Struct. Biol. 7, 384-388 (2000).

Assembly members:

Assembly members:
Cdc42, polymer, 185 residues, 20430.40 Da.
p21 activated kinase, polymer, 46 residues, 5127.64 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cdc42: MQTIKCVVVGDGAVGKTCLL ISYTTNKFPSEYVPTVFDNY AVTVMIGGEPYTLGLFDTAG LEDYDRLRPLSYPQTDVFLV CFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLR DDPSTIEKLAKNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFDEAILAALEPP EPKKX
p21 activated kinase: GSISLPSDFEHTIHVGFDAV TGEFTGMPEQWARLLQTSNI TKSEQK

Data sets:
Data typeCount
13C chemical shifts741
1H chemical shifts1550
15N chemical shifts224

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc421
2PAK2

Entities:

Entity 1, Cdc42 185 residues - 20430.40 Da.

1   METGLNTHRILELYSCYSVALVALVALGLY
2   ASPGLYALAVALGLYLYSTHRCYSLEULEU
3   ILESERTYRTHRTHRASNLYSPHEPROSER
4   GLUTYRVALPROTHRVALPHEASPASNTYR
5   ALAVALTHRVALMETILEGLYGLYGLUPRO
6   TYRTHRLEUGLYLEUPHEASPTHRALAGLY
7   LEUGLUASPTYRASPARGLEUARGPROLEU
8   SERTYRPROGLNTHRASPVALPHELEUVAL
9   CYSPHESERVALVALSERPROSERSERPHE
10   GLUASNVALLYSGLULYSTRPVALPROGLU
11   ILETHRHISHISCYSPROLYSTHRPROPHE
12   LEULEUVALGLYTHRGLNILEASPLEUARG
13   ASPASPPROSERTHRILEGLULYSLEUALA
14   LYSASNLYSGLNLYSPROILETHRPROGLU
15   THRALAGLULYSLEUALAARGASPLEULYS
16   ALAVALLYSTYRVALGLUCYSSERALALEU
17   THRGLNLYSGLYLEULYSASNVALPHEASP
18   GLUALAILELEUALAALALEUGLUPROPRO
19   GLUPROLYSLYSGNP

Entity 2, PAK 46 residues - 5127.64 Da.

1   GLYSERILESERLEUPROSERASPPHEGLU
2   HISTHRILEHISVALGLYPHEASPALAVAL
3   THRGLYGLUPHETHRGLYMETPROGLUGLN
4   TRPALAARGLEULEUGLNTHRSERASNILE
5   THRLYSSERGLUGLNLYS

Related Database Links:

BMRB 15424
PDB 1A4R 1AJE 1AM4 1AN0 1CEE 1CF4 1DOA 1E0A 1EES 1GRN 1GZS 1KI1 1KZ7 1KZG 1NF3 2ASE 2DFK 2KB0 2NGR 2ODB 2QRZ 2WM9 2WMN 2WMO 3EG5 3GCG 3QBV 3VHL 4DID 4ITR 4JS0 4YC7 4YDH 1F3M 2QME
DBJ BAB22563 BAC16312 BAC34669 BAC35825 BAE01909 BAC16311.1 BAC16312.1 BAF85787.1
EMBL CAA90215 CAB57325 CAB57326 CAB57327 CAB57328 CAF91245.1 CAN87991.1 CAN87992.1 CAF94511.1
GB AAA37410 AAA52494 AAA52592 AAB40051 AAC00027
REF NP_001003254 NP_001008027 NP_001017070 NP_001018130 NP_001034891 XP_001363384.1 XP_001363301.1 NP_035165.2 NP_001070366.1 XP_508657.2
SP P60766 P60952 P60953 Q007T2 Q2KJ93
TPG DAA32122
SWISS-PROT Q13153 O88643 Q08E52 P35465
PRF 2003404A
GenBank ACA13260.1 ACA13261.1 EAW75028.1 EDL16325.1 EDM18463.1
AlphaFold P60766 P60952 P60953 Q007T2 Q2KJ93

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