BMRB Entry 4492

Name: Unmyristoylated GCAP-2 with three calcium ions bound
Published: 2000-06-16

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PDB ID: 1jba
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4492
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Unmyristoylated GCAP-2 with three calcium ions bound

Deposition date:

1999-04-04

Original release date:

2000-06-16

Authors:

Ames, J.; Dizhoor, A.; Ikura, M.; Palczewski, K.; Stryer, L.

Citation:

Citation: Ames, J.; Dizhoor, A.; Ikura, M.; Palczewski, K.; Stryer, L.. "Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases" J. Biol. Chem. 274, 19329-19337 (1999).
PubMed: 10383444

Assembly members:

Assembly members:
guanylate cyclase activating protein 2, polymer, 204 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
guanylate cyclase activating protein 2: MGQQFSWEEAEENGAVGAAD AAQLQEWYKKFLEECPSGTL FMHEFKRFFKVPDNEEATQY VEAMFRAFDTNGDNTIDFLE YVAALNLVLRGTLEHKLKWT FKIYDKDRNGCIDRQELLDI VESIYKLKKACSVEVEAEQQ GKLLTPEEVVDRIFLLVDEN GDGQLSLNEFVEGARRDKWV MKMLQMDLNPSSWISQQRRK SAMF

Data sets:

assigned_chemical_shifts
- chem_shift_set_1

Data type	Count
1H chemical shifts	675
13C chemical shifts	558
15N chemical shifts	185

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GCAP-2	1
2	Ca2+ 1	2
3	Ca2+ 2	2
4	Ca2+ 3	2

Entities:

Entity 1, GCAP-2 204 residues - Formula weight is not available

1

MET

GLY

GLN

PHE

SER

TRP

GLU

ALA

2

GLU

ASN

GLY

ALA

VAL

GLY

ALA

ASP

3

ALA

GLN

LEU

GLN

GLU

TRP

TYR

LYS

4

PHE

LEU

GLU

CYS

PRO

SER

GLY

THR

LEU

5

PHE

MET

HIS

GLU

PHE

LYS

ARG

PHE

LYS

6

VAL

PRO

ASP

ASN

GLU

ALA

THR

GLN

TYR

7

VAL

GLU

ALA

MET

PHE

ARG

ALA

PHE

ASP

THR

8

ASN

GLY

ASP

ASN

THR

ILE

ASP

PHE

LEU

GLU

9

TYR

VAL

ALA

LEU

ASN

LEU

VAL

LEU

ARG

10

GLY

THR

LEU

GLU

HIS

LYS

LEU

LYS

TRP

THR

11

PHE

LYS

ILE

TYR

ASP

LYS

ASP

ARG

ASN

GLY

12

CYS

ILE

ASP

ARG

GLN

GLU

LEU

ASP

ILE

13

VAL

GLU

SER

ILE

TYR

LYS

LEU

LYS

ALA

14

CYS

SER

VAL

GLU

VAL

GLU

ALA

GLU

GLN

15

GLY

LYS

LEU

THR

PRO

GLU

VAL

16

ASP

ARG

ILE

PHE

LEU

VAL

ASP

GLU

ASN

17

GLY

ASP

GLY

GLN

LEU

SER

LEU

ASN

GLU

PHE

18

VAL

GLU

GLY

ALA

ARG

ASP

LYS

TRP

VAL

19

MET

LYS

MET

LEU

GLN

MET

ASP

LEU

ASN

PRO

20

SER

TRP

ILE

SER

GLN

ARG

LYS

21

SER

ALA

MET

PHE

Entity 2, Ca2+ 1 - Ca - 40.078 Da.

1

CA

Samples:

sample_1: guanylate cyclase activating protein 2, [U-15N], 1 mM; KCl 50 mM; CaCl2 10 mM; [2H10]dithiothreitol 10 mM; [2H28]octyl-glucoside 25 mM

sample_2: guanylate cyclase activating protein 2, [U-13C; U-15N], 1 mM; KCl 50 mM; CaCl2 10 mM; [2H10]dithiothreitol 10 mM; [2H28]octyl-glucoside 25 mM

sample_cond_1: pH: 6.8; temperature: 318 K; ionic strength: 0.1 M; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
HNCO	not available	not available	sample_cond_1
HNCACB	not available	not available	sample_cond_1
CBCACONH	not available	not available	sample_cond_1
NOESY	not available	not available	sample_cond_1
TOCSY	not available	not available	sample_cond_1

Software:

No software information available

NMR spectrometers:

Varian Unity-Plus 500 MHz

BMRB	25199
PDB	1JBA
GB	AAA83214 AAC48478 ELR53264
REF	NP_777211 XP_005900200 XP_006069256 XP_010816471 XP_010861473
SP	P51177
TPG	DAA16519
AlphaFold	P51177