BMRB Entry 25199

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25199

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Title:
Backbone (HN, NH, CO and CA) Chemical Shift Assignments for Ca(2+)-bound Myristoylated GCAP-2 in the Presence of 10 mM CHAPS
Deposition date:
2014-09-04
Original release date:
2014-10-07
Authors:
Margetic, Aleksandra; Nannemann, David; Meiler, Jens; Huster, Daniel; Theisgen, Stephan
Citation:

Citation: Margetic, Aleksandra; Nannemann, David; Meiler, Jens; Huster, Daniel; Theisgen, Stephan. "Guanylate Cyclase-Activating Protein-2 Undergoes Structural Changes upon Binding to Detergent Micelles and Bicelles"  Biochim. Biophys. Acta - Biomembranes 1838, 2767-2777 (2014).
PubMed: 25051529

Assembly members:

Assembly members:
myrGCAP2, polymer, 203 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: domestic cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
myrGCAP2: GQQFSWEEAEENGAVGAADA AQLQEWYKKFLEECPSGTLF MHEFKRFFKVPDNEEATQYV EAMFRAFDTNGDNTIDFLEY VAALNLVLRGTLEHKLKWTF KIYDKDRNGCIDRQELLDIV ESIYKLKKACSVEVEAEQQG KLLTPEEVVDRIFLLVDENG DGQLSLNEFVEGARRDKWVM KMLQMDLNPSSWISQQRRKS AMF

Data sets:
Data typeCount
1H chemical shifts167
13C chemical shifts356
15N chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myrGCAP-21
2Ca2+ - 12
3Ca2+ - 22
4Ca2+ - 32

Entities:

Entity 1, myrGCAP-2 203 residues - Formula weight is not available

due to myristoylation sequence starts at residue 2

1   GLYGLNGLNPHESERTRPGLUGLUALAGLU
2   GLUASNGLYALAVALGLYALAALAASPALA
3   ALAGLNLEUGLNGLUTRPTYRLYSLYSPHE
4   LEUGLUGLUCYSPROSERGLYTHRLEUPHE
5   METHISGLUPHELYSARGPHEPHELYSVAL
6   PROASPASNGLUGLUALATHRGLNTYRVAL
7   GLUALAMETPHEARGALAPHEASPTHRASN
8   GLYASPASNTHRILEASPPHELEUGLUTYR
9   VALALAALALEUASNLEUVALLEUARGGLY
10   THRLEUGLUHISLYSLEULYSTRPTHRPHE
11   LYSILETYRASPLYSASPARGASNGLYCYS
12   ILEASPARGGLNGLULEULEUASPILEVAL
13   GLUSERILETYRLYSLEULYSLYSALACYS
14   SERVALGLUVALGLUALAGLUGLNGLNGLY
15   LYSLEULEUTHRPROGLUGLUVALVALASP
16   ARGILEPHELEULEUVALASPGLUASNGLY
17   ASPGLYGLNLEUSERLEUASNGLUPHEVAL
18   GLUGLYALAARGARGASPLYSTRPVALMET
19   LYSMETLEUGLNMETASPLEUASNPROSER
20   SERTRPILESERGLNGLNARGARGLYSSER
21   ALAMETPHE

Entity 2, Ca2+ - 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: myrGCAP2, [U-100% 13C; U-100% 15N], 0.2 – 0.5 mM; CaCl2 10 ± 0.5 mM; CHAPS 10 ± 0.5 mM; MES 20 ± 0.5 mM; TCEP 2 ± 0.5 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 323 K; pH: 6.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4492
PDB
GB AAA83214 AAC48478 ELR53264
REF NP_777211 XP_005900200 XP_006069256 XP_010816471 XP_010861473
SP P51177
TPG DAA16519
AlphaFold P51177

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks