BMRB Entry 4306

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4306

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Title:
Backbone NMR asignment of a cyanobacterial transcriptional factor, SmtB, that has bound zinc ions
Deposition date:
1999-02-03
Original release date:
2000-07-14
Authors:
Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa
Citation:

Citation: Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa. "Backbone NMR assignments of a cyanobacterial transcriptional factor, SmtB, that binds zinc ions"  J. Biomol. NMR 14, 191-192 (1999).

Assembly members:

Assembly members:
transcriptional repressor, polymer, 121 residues, 13395.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: T-21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
transcriptional repressor: TKPVLQDGETVVCQGTHAAI ASELQAIAPEVAQSLAEFFA VLADPNRLRLLSLLARSELC VGDLAQAIGVSESAVSHQLR SLRNLRLVSYRKQGRHVYYQ LQDHHIVALYQNALDHLQEC R

Data sets:
Data typeCount
13C chemical shifts119
15N chemical shifts114
1H chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SmtB subunit 11
2SmtB subunit 21
3Zn12
4Zn22
5Zn32
6Zn42

Entities:

Entity 1, SmtB subunit 1 121 residues - 13395.2 Da.

1   THRLYSPROVALLEUGLNASPGLYGLUTHR
2   VALVALCYSGLNGLYTHRHISALAALAILE
3   ALASERGLULEUGLNALAILEALAPROGLU
4   VALALAGLNSERLEUALAGLUPHEPHEALA
5   VALLEUALAASPPROASNARGLEUARGLEU
6   LEUSERLEULEUALAARGSERGLULEUCYS
7   VALGLYASPLEUALAGLNALAILEGLYVAL
8   SERGLUSERALAVALSERHISGLNLEUARG
9   SERLEUARGASNLEUARGLEUVALSERTYR
10   ARGLYSGLNGLYARGHISVALTYRTYRGLN
11   LEUGLNASPHISHISILEVALALALEUTYR
12   GLNASNALALEUASPHISLEUGLNGLUCYS
13   ARG

Entity 2, Zn1 - Zn - 65.409 Da.

1   ZN

Related Database Links:

BMRB 4128
PDB 1R1T 1R22 1R23 1SMT
DBJ BAD78452
EMBL CAA45872
GB AAT01609 AAT01610 AAT01611 AAT01612 AAT01613
REF WP_011242576
SP P30340
AlphaFold P30340

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks