BMRB Entry 4300

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PDB ID: 2f65
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4300
MolProbity Validation Chart

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Title:
1H , 13C, 15N Assigned Chemical Shifts for HPPK-AMPPCP Complex
Deposition date:
1999-01-22
Original release date:
1999-08-12
Authors:
Shi, Genbin; Gao, Jinhai; Yan, Honggao
Citation:

Citation: Shi, Genbin; Gao, Jinhai; Yan, Honggao. "1H, 13C and 15N Resonance Assignments of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin Pyrophosphokinase and its Complex with MgAMPPCP"  J. Biomol. NMR 14, 189-190 (1999).

Assembly members:

Assembly members:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, polymer, 158 residues, Formula weight is not available
beta gamma-methyleneadenosine 5'triphosphate, non-polymer, Formula weight is not available
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: TVAYIAIGSNLASPLEQVNA ALKALGDIPESHILTVSSFY RTPPLGPQDQPDYLNAAVAL ETSLAPEELLNHTQRIELQQ GRVRKAERWGPRTLDLDIML FGNEVINTERLTVPHYDMKN RGFMLWPLFEIAPELVFPDG EMLRQILHTRAFDKLNKW

Data sets:
Data typeCount
1H chemical shifts868
13C chemical shifts240
15N chemical shifts139

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPPK monomer1
2AMPPCP2
3Mg3

Entities:

Entity 1, HPPK monomer 158 residues - Formula weight is not available

1   THRVALALATYRILEALAILEGLYSERASN
2   LEUALASERPROLEUGLUGLNVALASNALA
3   ALALEULYSALALEUGLYASPILEPROGLU
4   SERHISILELEUTHRVALSERSERPHETYR
5   ARGTHRPROPROLEUGLYPROGLNASPGLN
6   PROASPTYRLEUASNALAALAVALALALEU
7   GLUTHRSERLEUALAPROGLUGLULEULEU
8   ASNHISTHRGLNARGILEGLULEUGLNGLN
9   GLYARGVALARGLYSALAGLUARGTRPGLY
10   PROARGTHRLEUASPLEUASPILEMETLEU
11   PHEGLYASNGLUVALILEASNTHRGLUARG
12   LEUTHRVALPROHISTYRASPMETLYSASN
13   ARGGLYPHEMETLEUTRPPROLEUPHEGLU
14   ILEALAPROGLULEUVALPHEPROASPGLY
15   GLUMETLEUARGGLNILELEUHISTHRARG
16   ALAPHEASPLYSLEUASNLYSTRP

Entity 2, AMPPCP 1 residues - C11H16N5O12P3Na2 - Formula weight is not available

1   AMPPCP

Entity 3, Mg - Mg - 24.305 Da.

1   MG

Related Database Links:

BMRB 4299
PDB 1DY3 1EQ0 1EQM 1EX8 1F9H 1G4C 1HKA 1HQ2 1IM6 1KBR 1Q0N 1RAO 1RB0 1TMJ 1TMM 2F63 2F65 3HCX 3HD1 3HD2 3HSD 3HSG 3HSJ 3HSZ 3HT0 3ILI 3ILJ 3ILL 3ILO 3IP0 3KUE 3KUG 3KUH 3UD5 3UDE 3UDV 4F7V 4M5G 4M5H 4M5I 4M5J 4M5K 4M5L 4M5M 4M5N
DBJ BAB96719 BAJ41992 BAL37452
EMBL CBI99639 CCP97244 CDJ70723 CDK83585 CDY54783
GB AAB53446 AAC73253 ACA79131 ACB01321 ACR61761
REF NP_414684 WP_000215127 WP_000215131 WP_000215137 WP_000215138
SP P26281
AlphaFold P26281

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