BMRB Entry 4097

Name: Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain
Published: 1999-01-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4097

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Chemical Shift Assignment for the HIV-1 RNase H Domain

Deposition date:

1998-01-16

Original release date:

1999-01-25

Authors:

Gunther, Kern; Handel, Tracy; Marqusee, Susan

Citation:

Citation: Gunther, Kern; Handel, Tracy; Marqusee, Susan. "Characterization of a Folding Intermediate from HIV-1 Ribonuclease H" Protein Sci. 7, 2164-2174 (1998).

Assembly members:

Assembly members:
Reverse Transcriptase of HIV-1, polymer, 135 residues, 14885 Da.

Natural source:

Natural source: Common Name: HIV-1 Taxonomy ID: 11678 Superkingdom: not available Kingdom: not available Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source: Production method: chemical construct Vector: pAED4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Reverse Transcriptase of HIV-1: MYQLEKEPIVGAETFYVDGA ANRETKLGKAGYVTNKGRQK VVPLTNTTNQKTELQAIYLA LQDSGLEVNIVTDSQYALGI IQAQPDKSESELVNQIIEQL IKKEKVYLAWVPAHKGIGGN EQVDKLVSAGIRKIL

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	217
15N chemical shifts	111
1H chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNase_H-HIV-1_(RVT)	1

Entities:

Entity 1, RNase_H-HIV-1_(RVT) 135 residues - 14885 Da.

1

MET

TYR

GLN

LEU

GLU

LYS

GLU

PRO

ILE

VAL

2

GLY

ALA

GLU

THR

PHE

TYR

VAL

ASP

GLY

ALA

3

ALA

ASN

ARG

GLU

THR

LYS

LEU

GLY

LYS

ALA

4

GLY

TYR

VAL

THR

ASN

LYS

GLY

ARG

GLN

LYS

5

VAL

PRO

LEU

THR

ASN

THR

ASN

GLN

6

LYS

THR

GLU

LEU

GLN

ALA

ILE

TYR

LEU

ALA

7

LEU

GLN

ASP

SER

GLY

LEU

GLU

VAL

ASN

ILE

8

VAL

THR

ASP

SER

GLN

TYR

ALA

LEU

GLY

ILE

9

ILE

GLN

ALA

GLN

PRO

ASP

LYS

SER

GLU

SER

10

GLU

LEU

VAL

ASN

GLN

ILE

GLU

GLN

LEU

11

ILE

LYS

GLU

LYS

VAL

TYR

LEU

ALA

TRP

12

VAL

PRO

ALA

HIS

LYS

GLY

ILE

GLY

ASN

13

GLU

GLN

VAL

ASP

LYS

LEU

VAL

SER

ALA

GLY

14

ILE

ARG

LYS

ILE

LEU

Samples:

sample_one: Reverse Transcriptase of HIV-1, [U-99.8% 13C; U-99.9% 15N], 1.1 mM

sample_conditions_one: ionic strength: 0.1; pH: 4.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_one	not available	not available
HNCA	sample_one	not available	not available
CBCA(CO)NH	sample_one	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DMX600 600 MHz

Related Database Links:

PDB	1BQM 1BQN 1DLO 1EET 1HMV 1HNI 1HNV 1HPZ 1HQE 1HQU 1HRH 1HVU 1HYS 1IKV 1IKW 1IKX 1IKY 1J5O 1N5Y 1N6Q 1QE1 1R0A 1RDH 1RTD 1S6P 1S6Q 1S9E 1S9G 1SUQ 1SV5 1T03 1T05 1TV6 1TVR 1UWB 2B5J 2B6A 2BAN 2BE2 2HMI 2I5J 2IAJ 2IC3 2VG5 2VG6 2VG7 2YKM 2YKN 2ZD1 2ZE2 3BGR 3DLK 3HVT 3IG1 3IRX 3IS9 3ISN 3ITH 3JSM 3JYT 3K2P 3KLE 3KLF 3KLG 3KLH 3KLI 3LP3 3QLH 3QO9 3V4I 3V6D 3V81 4DG1 4G1Q 4H4M 4H4O 4I2P 4I2Q 4ICL 4ID5 4IDK 4IFV 4IFY 4IG0 4IG3 4KFB 4KKO 4KO0 4LSL 4LSN 4MFB 4O44 4O4G 4PQU 4PUO 4PWD 4Q0B 4QAG 4R5P 4RW4 4RW6 4RW7 4RW8 4RW9 4WE1 5C24 5C25 5C42 5D3G
DBJ	BAA12989
GB	AAA44198 AAB24839 AAL28068 AAL29457 ADW78894
PRF	1102247C 1103299E
SP	P03366 P0C6F2
AlphaFold	P03366 P0C6F2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks