BMRB Entry 36202

Name: Solution Structure for helix 45 in 3&#039; end of 12S rRNA
Published: 2025-09-27

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PDB ID: 6aas
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36202
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure for helix 45 in 3' end of 12S rRNA

Deposition date:

2018-07-19

Original release date:

2025-09-27

Authors:

Liu, X.; Wu, P.

Citation:

Citation: Liu, Xiaodan; Shen, Shengqi; Wu, Pengzhi; Li, Fudong; Liu, Xiaodan; Wang, Chongyuan; Gong, Qingguo; Wu, Jihui; Yao, Xuebiao; Zhang, Huafeng; Shi, Yunyu. "Structural insights into dimethylation of 12S rRNA by TFB1M: indispensable role in translation of mitochondrial genes and mitochondrial function." Nucleic Acids Res. 47, 7648-7665 (2019).
PubMed: 31251801

Assembly members:

Assembly members:
RNA (28-MER), polymer, 28 residues, 9035.406 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method:

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA (28-MER): GGUAAGUGUACUGGAAAGUG CACUUGCC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	165
15N chemical shifts	25
1H chemical shifts	201

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 28 residues - 9035.406 Da.

1

G

U

A

G

U

G

U

A

2

C

U

G

A

G

U

G

3

C

A

C

U

G

C

Samples:

15N_13C_sample: RNA (28-MER), [U-13C; U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_2: RNA (28-MER), [U-13C; U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_3: RNA (28-MER), [U-13C; U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_4: RNA (28-MER), [U-13C; U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_5: RNA (28-MER), [U-13C; U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 2 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HNN-COSY	15N_13C_sample	isotropic	sample_conditions_1
1H-15N HSQC	15N_13C_sample	isotropic	sample_conditions_1
1H-13C CT-HSQC	15N_13C_sample	isotropic	sample_conditions_1
3D 13C NOESY-HSQC	15N_13C_sample	isotropic	sample_conditions_1
13C HCCH-TOCSY	15N_13C_sample	isotropic	sample_conditions_1

Software:

Amber, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

Sparky, Goddard - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

Bruker AVANCE 600 MHz