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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27664
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Condos, Tara; Shaw, Gary. "Synergistic Recruitment of UbcH7~Ub and Phosphorylated Ubl Domain Triggers Parkin Activation" EMBO J. ., .-..
Assembly members:
UbcH7, polymer, 154 residues, Formula weight is not available
Ub, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Data type | Count |
15N chemical shifts | 208 |
1H chemical shifts | 196 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | UbcH7 | 1 |
2 | Ub | 2 |
Entity 1, UbcH7 154 residues - Formula weight is not available
1 | MET | ALA | ALA | SER | ARG | ARG | LEU | MET | LYS | GLU | ||||
2 | LEU | GLU | GLU | ILE | ARG | LYS | SER | GLY | MET | LYS | ||||
3 | ASN | PHE | ARG | ASN | ILE | GLN | VAL | ASP | GLU | ALA | ||||
4 | ASN | LEU | LEU | THR | TRP | GLN | GLY | LEU | ILE | VAL | ||||
5 | PRO | ASP | ASN | PRO | PRO | TYR | ASP | LYS | GLY | ALA | ||||
6 | PHE | ARG | ILE | GLU | ILE | ASN | PHE | PRO | ALA | GLU | ||||
7 | TYR | PRO | PHE | LYS | PRO | PRO | LYS | ILE | THR | PHE | ||||
8 | LYS | THR | LYS | ILE | TYR | HIS | PRO | ASN | ILE | ASP | ||||
9 | GLU | LYS | GLY | GLN | VAL | LYS | LEU | PRO | VAL | ILE | ||||
10 | SER | ALA | GLU | ASN | TRP | LYS | PRO | ALA | THR | LYS | ||||
11 | THR | ASP | GLN | VAL | ILE | GLN | SER | LEU | ILE | ALA | ||||
12 | LEU | VAL | ASN | ASP | PRO | GLN | PRO | GLU | HIS | PRO | ||||
13 | LEU | ARG | ALA | ASP | LEU | ALA | GLU | GLU | TYR | SER | ||||
14 | LYS | ASP | ARG | LYS | LYS | PHE | SER | LYS | ASN | ALA | ||||
15 | GLU | GLU | PHE | THR | LYS | LYS | TYR | GLY | GLU | LYS | ||||
16 | ARG | PRO | VAL | ASP |
Entity 2, Ub 76 residues - Formula weight is not available
1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | SER | SER | ||||
3 | ASP | THR | ILE | ASP | ASN | VAL | LYS | SER | LYS | ILE | ||||
4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
8 | LEU | ARG | LEU | ARG | GLY | GLY |
sample_1: UbcH7, [U-99% 2H], 0.11 ± 0.1 mM; Ub, [U-99% 2H], 0.11 ± 0.1 mM; DSS 0.01 mM; HEPES 25 ± 0.1 mM; sodium chloride 50 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
NMRPipe v9.2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRViewJ v9.2.0, Johnson, One Moon Scientific - chemical shift assignment, peak picking
VNMRJ v4.2, Varian - collection
NMRView v9.2.0, Johnson, One Moon Scientific - chemical shift assignment, peak picking
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