BMRB Entry 15498

Name: Backbone and side-chain 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH7
Published: 2008-01-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15498

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH7

Deposition date:

2007-09-27

Original release date:

2008-01-17

Authors:

Serniwka, Stephanie; Shaw, Gary

Citation:

Citation: Serniwka, Stephanie; Shaw, Gary. "1H, 13C and 15N resonance assignments for the human E2 conjugating enzyme, UbcH7" Biomol. NMR Assignments 2, 21-23 (2008).
PubMed: 19636915

Assembly members:

Assembly members:
UbcH7, polymer, 153 residues, 18684 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UbcH7: AASRRLMKELEEIRKSGMKN FRNIQVDEANLLTWQGLIVP DNPPYDKGAFRIEINFPAEY PFKPPKITFKTKIYHPNIDE KGQVCLPVISAENWKPATKT DQVIQSLIALVNDPQPEHPL RADLAEEYSKDRKKFSKNAE EFTKKYGEKRPVD

Data sets:

assigned_chemical_shifts
- Assigned_Chemical_Shift_List_for_UbcH7

Data type	Count
13C chemical shifts	602
15N chemical shifts	150
1H chemical shifts	736

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UbcH7	1

Entities:

Entity 1, UbcH7 153 residues - 18684 Da.

1

ALA

SER

ARG

LEU

MET

LYS

GLU

LEU

2

GLU

ILE

ARG

LYS

SER

GLY

MET

LYS

ASN

3

PHE

ARG

ASN

ILE

GLN

VAL

ASP

GLU

ALA

ASN

4

LEU

THR

TRP

GLN

GLY

LEU

ILE

VAL

PRO

5

ASP

ASN

PRO

TYR

ASP

LYS

GLY

ALA

PHE

6

ARG

ILE

GLU

ILE

ASN

PHE

PRO

ALA

GLU

TYR

7

PRO

PHE

LYS

PRO

LYS

ILE

THR

PHE

LYS

8

THR

LYS

ILE

TYR

HIS

PRO

ASN

ILE

ASP

GLU

9

LYS

GLY

GLN

VAL

CYS

LEU

PRO

VAL

ILE

SER

10

ALA

GLU

ASN

TRP

LYS

PRO

ALA

THR

LYS

THR

11

ASP

GLN

VAL

ILE

GLN

SER

LEU

ILE

ALA

LEU

12

VAL

ASN

ASP

PRO

GLN

PRO

GLU

HIS

PRO

LEU

13

ARG

ALA

ASP

LEU

ALA

GLU

TYR

SER

LYS

14

ASP

ARG

LYS

PHE

SER

LYS

ASN

ALA

GLU

15

GLU

PHE

THR

LYS

TYR

GLY

GLU

LYS

ARG

16

PRO

VAL

ASP

Samples:

sample: UbcH7, [U-100% 13C; U-100% 15N], 0.5 mM; TRIS 25 mM; EDTA 1 mM; DTT 5 mM; sodium chloride 200 mM

Sample_Conditions_for_UbcH7: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample	isotropic	Sample_Conditions_for_UbcH7
2D 1H-13C HSQC	sample	isotropic	Sample_Conditions_for_UbcH7
3D HCCH-TOCSY	sample	isotropic	Sample_Conditions_for_UbcH7
3D CBCA(CO)NH	sample	isotropic	Sample_Conditions_for_UbcH7
3D C(CO)NH	sample	isotropic	Sample_Conditions_for_UbcH7
3D HNCO	sample	isotropic	Sample_Conditions_for_UbcH7
3D HNCACB	sample	isotropic	Sample_Conditions_for_UbcH7
3D H(CCO)NH	sample	isotropic	Sample_Conditions_for_UbcH7

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz

PDB	1C4Z 1FBV 3SQV 3SY2 4Q5E 4Q5H
DBJ	BAB24925 BAE38300 BAE39867 BAE87197 BAF83772
EMBL	CAA04156 CAA10265 CAA63538 CAA65755 CAG30492
GB	AAB36017 AAG17922 AAH53368 AAH77940 AAH93503
REF	NP_001006180 NP_001071562 NP_001086444 NP_001127614 NP_001172062
SP	P68036 P68037 Q3MHP1 Q5R5I4
TPG	DAA20415
AlphaFold	P68036 P68037 Q3MHP1 Q5R5I4