BMRB Entry 25552

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25552
MolProbity Validation Chart

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Title:
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication - NMR solution structure of human Polo-box 3
Deposition date:
2015-03-24
Original release date:
2015-08-07
Authors:
Boehm, Raphael; Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Sauer, Evelyn; Nigg, Erich; Maier, Timm; Hiller, Sebastian
Citation:

Citation: Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Boehm, Raphael; Sauer, Evelyn; Hiller, Sebastian; Nigg, Erich; Maier, Timm. "STIL binding to Polo-box 3 of PLK4 regulates centriole duplication."  eLife 4, .-. (2015).
PubMed: 26188084

Assembly members:

Assembly members:
entity, polymer, 88 residues, 9629.920 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETG-30A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GSAQLLKSVFVKNVGWATQL TSGAVWVQFNDGSQLVVQAG VSSISYTSPNGQTTRYGENE KLPDYIKQKLQCLSSILLMF SNPTPNFH

Data sets:
Data typeCount
13C chemical shifts266
15N chemical shifts82
1H chemical shifts192

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 88 residues - 9629.920 Da.

1   GLYSERALAGLNLEULEULYSSERVALPHE
2   VALLYSASNVALGLYTRPALATHRGLNLEU
3   THRSERGLYALAVALTRPVALGLNPHEASN
4   ASPGLYSERGLNLEUVALVALGLNALAGLY
5   VALSERSERILESERTYRTHRSERPROASN
6   GLYGLNTHRTHRARGTYRGLYGLUASNGLU
7   LYSLEUPROASPTYRILELYSGLNLYSLEU
8   GLNCYSLEUSERSERILELEULEUMETPHE
9   SERASNPROTHRPROASNPHEHIS

Samples:

Deuterated-triple_labelled_PB3: PLK4-PB3, [U-2H; U-13C; U-15N], 0.6 – 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%

ILV_PB3_NOESY-type: PLK4-PB3, [U-2H; U-15N; ILV(methyl)-13C,1H], 0.6 – 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%

ILV_PB3_TOCSY-type: PLK4-PB3, [U-2H; U-13C; U-15N; ILV(methyl)-1H], 1.2 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%

Deuterated-15N_PB3: PLK4-PB3, [U-2H; U-15N], 0.6 uM; MOPS 20 mM; sodium chloride 30 mM; TCEP 2 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 30 mM; pH: 7; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCDeuterated-triple_labelled_PB3isotropicsample_conditions_1
3D HNCODeuterated-triple_labelled_PB3isotropicsample_conditions_1
3D HNCADeuterated-triple_labelled_PB3isotropicsample_conditions_1
3D HNCACBDeuterated-triple_labelled_PB3isotropicsample_conditions_1
3D HNCACODeuterated-triple_labelled_PB3isotropicsample_conditions_1
3D 1H-15N NOESYILV_PB3_NOESY-typeisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticILV_PB3_NOESY-typeisotropicsample_conditions_1
3D (H)C(CC)-TOCSY-(CO)-15N-HSQCILV_PB3_TOCSY-typeisotropicsample_conditions_1
3D H(C)(CC)-TOCSY-(CO)-15N-HSQCILV_PB3_TOCSY-typeisotropicsample_conditions_1
15N-filtered BPP-LED NMRDeuterated-15N_PB3isotropicsample_conditions_1
15N-{1H}-Heteronuclear NOEDeuterated-15N_PB3isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticILV_PB3_TOCSY-typeisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, data analysis, peak picking, structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 26547
PDB
DBJ BAB24599 BAB69958 BAE02419 BAE23231 BAE23367
EMBL CAA73575 CAH91413
GB AAC37648 AAH26785 AAH36023 AAH57940 AAX43264
REF NP_001153257 NP_001177728 NP_001177730 NP_035625 NP_055079
SP O00444 Q5R9Z7 Q64702
AlphaFold O00444 Q5R9Z7 Q64702

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks