BMRB Entry 26547
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26547
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Boehm, Raphael; Sauer, Evelyn; Hiller, Sebastian; Nigg, Erich; Maier, Timm. "STIL binding to Polo-box 3 of PLK4 regulates centriole duplication." eLife 4, .-. (2015).
PubMed: 26188084
Assembly members:
PLK4-PB3, polymer, 88 residues, Formula weight is not available
PLK4-PB3, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETG-30A
Entity Sequences (FASTA):
PLK4-PB3: GSAQLLKSVFVKNVGWATQL
TSGAVWVQFNDGSQLVVQAG
VSSISYTSPNGQTTRYGENE
KLPDYIKQKLQCLSSILLMF
SNPTPNFH
PLK4-PB3: PDAYRFLTEQDRQLRLLQAQ
IQRLLEAQSLMP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 154 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PLK4-PB3 | 1 |
| 2 | STIL-CC | 2 |
Entities:
Entity 1, PLK4-PB3 88 residues - Formula weight is not available
| 1 | GLY | SER | ALA | GLN | LEU | LEU | LYS | SER | VAL | PHE | ||||
| 2 | VAL | LYS | ASN | VAL | GLY | TRP | ALA | THR | GLN | LEU | ||||
| 3 | THR | SER | GLY | ALA | VAL | TRP | VAL | GLN | PHE | ASN | ||||
| 4 | ASP | GLY | SER | GLN | LEU | VAL | VAL | GLN | ALA | GLY | ||||
| 5 | VAL | SER | SER | ILE | SER | TYR | THR | SER | PRO | ASN | ||||
| 6 | GLY | GLN | THR | THR | ARG | TYR | GLY | GLU | ASN | GLU | ||||
| 7 | LYS | LEU | PRO | ASP | TYR | ILE | LYS | GLN | LYS | LEU | ||||
| 8 | GLN | CYS | LEU | SER | SER | ILE | LEU | LEU | MET | PHE | ||||
| 9 | SER | ASN | PRO | THR | PRO | ASN | PHE | HIS |
Entity 2, STIL-CC 88 residues - Formula weight is not available
| 1 | PRO | ASP | ALA | TYR | ARG | PHE | LEU | THR | GLU | GLN | ||||
| 2 | ASP | ARG | GLN | LEU | ARG | LEU | LEU | GLN | ALA | GLN | ||||
| 3 | ILE | GLN | ARG | LEU | LEU | GLU | ALA | GLN | SER | LEU | ||||
| 4 | MET | PRO |
Samples:
Deuterated-triple_labelled_PLK4-PB3-STIL-CC: PLK4-PB3, [U-13C; U-15N; U-2H], 800 uM; SCL/TAL1 INTERRUPTING LOCUS PROTEIN-COILED COIL REGION (STIL-CC) 800 uM; sodium chloride 30 mM; TCEP 2 mM; MOPS 20 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0.03 M; pH: 7; pressure: 1 atm; temperature: 293.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | Deuterated-triple_labelled_PLK4-PB3-STIL-CC | isotropic | sample_conditions_1 |
| 3D HNCO | Deuterated-triple_labelled_PLK4-PB3-STIL-CC | isotropic | sample_conditions_1 |
| 3D HNCA | Deuterated-triple_labelled_PLK4-PB3-STIL-CC | isotropic | sample_conditions_1 |
| 3D HNCACB | Deuterated-triple_labelled_PLK4-PB3-STIL-CC | isotropic | sample_conditions_1 |
| 3D HNCACO | Deuterated-triple_labelled_PLK4-PB3-STIL-CC | isotropic | sample_conditions_1 |
Software:
CARA, Rochus Keller - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Related Database Links:
| BMRB | 25552 |
| PDB | |
| DBJ | BAB24599 BAB69958 BAE02419 BAE23231 BAE23367 |
| EMBL | CAA73575 CAH91413 |
| GB | AAC37648 AAH26785 AAH36023 AAH57940 AAX43264 |
| REF | NP_001153257 NP_001177728 NP_001177730 NP_035625 NP_055079 |
| SP | O00444 Q5R9Z7 Q64702 |
| AlphaFold | O00444 Q5R9Z7 Q64702 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks