BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 26547

Title: STIL binding to the Polo-box 3 of PLK4 regulates centriole duplication - Backbone assignment of human Polo-box 3 bound to STIL   PubMed: 26188084

Deposition date: 2015-03-23 Original release date: 2015-08-07

Authors: Boehm, Raphael; Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Sauer, Evelyn; Nigg, Erich; Maier, Timm; Hiller, Sebastian

Citation: Arquint, Christian; Gabryjonczyk, Anna-Maria; Imseng, Stefan; Boehm, Raphael; Sauer, Evelyn; Hiller, Sebastian; Nigg, Erich; Maier, Timm. "STIL binding to Polo-box 3 of PLK4 regulates centriole duplication."  eLife 4, .-. (2015).

Assembly members:
PLK4-PB3, polymer, 88 residues, Formula weight is not available
PLK4-PB3, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETG-30A

Entity Sequences (FASTA):
PLK4-PB3: GSAQLLKSVFVKNVGWATQL TSGAVWVQFNDGSQLVVQAG VSSISYTSPNGQTTRYGENE KLPDYIKQKLQCLSSILLMF SNPTPNFH
PLK4-PB3: PDAYRFLTEQDRQLRLLQAQ IQRLLEAQSLMP

Data sets:
Data typeCount
13C chemical shifts154
15N chemical shifts70
1H chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PLK4-PB31
2STIL-CC2

Entities:

Entity 1, PLK4-PB3 88 residues - Formula weight is not available

1   GLYSERALAGLNLEULEULYSSERVALPHE
2   VALLYSASNVALGLYTRPALATHRGLNLEU
3   THRSERGLYALAVALTRPVALGLNPHEASN
4   ASPGLYSERGLNLEUVALVALGLNALAGLY
5   VALSERSERILESERTYRTHRSERPROASN
6   GLYGLNTHRTHRARGTYRGLYGLUASNGLU
7   LYSLEUPROASPTYRILELYSGLNLYSLEU
8   GLNCYSLEUSERSERILELEULEUMETPHE
9   SERASNPROTHRPROASNPHEHIS

Entity 2, STIL-CC 88 residues - Formula weight is not available

1   PROASPALATYRARGPHELEUTHRGLUGLN
2   ASPARGGLNLEUARGLEULEUGLNALAGLN
3   ILEGLNARGLEULEUGLUALAGLNSERLEU
4   METPRO

Samples:

Deuterated-triple_labelled_PLK4-PB3-STIL-CC: PLK4-PB3, [U-13C; U-15N; U-2H], 800 uM; SCL/TAL1 INTERRUPTING LOCUS PROTEIN-COILED COIL REGION (STIL-CC) 800 uM; sodium chloride 30 mM; TCEP 2 mM; MOPS 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.03 M; pH: 7; pressure: 1 atm; temperature: 293.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCDeuterated-triple_labelled_PLK4-PB3-STIL-CCisotropicsample_conditions_1
3D HNCODeuterated-triple_labelled_PLK4-PB3-STIL-CCisotropicsample_conditions_1
3D HNCADeuterated-triple_labelled_PLK4-PB3-STIL-CCisotropicsample_conditions_1
3D HNCACBDeuterated-triple_labelled_PLK4-PB3-STIL-CCisotropicsample_conditions_1
3D HNCACODeuterated-triple_labelled_PLK4-PB3-STIL-CCisotropicsample_conditions_1

Software:

CARA, Rochus Keller - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 25552
PDB
DBJ BAB24599 BAB69958 BAE02419 BAE23231 BAE23367
EMBL CAA73575 CAH91413
GB AAC37648 AAH26785 AAH36023 AAH57940 AAX43264
REF NP_001153257 NP_001177728 NP_001177730 NP_035625 NP_055079
SP O00444 Q5R9Z7 Q64702

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts