BMRB Entry 25101

Name: Solution NMR structure of tRNApro:MLV Nucleocapsid Protein (1:1) Complex
Published: 2014-09-08

Click here to enlarge.

PDB ID: 2ms1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25101
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution NMR structure of tRNApro:MLV Nucleocapsid Protein (1:1) Complex

Deposition date:

2014-07-19

Original release date:

2014-09-08

Authors:

D'Souza, Victoria; Yildiz, Zehra

Citation:

Citation: Miller, Sarah; Yildiz, F.; Lo, Jennifer; Wang, Bo; D'Souza, Victoria. "A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing" Nature 515, 591-595 (2014).
PubMed: 25209668

Assembly members:

Assembly members:
MLV_Nucleocapsid, polymer, 55 residues, 6264.138 Da.
tRNApro, polymer, 71 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Murine Leukemia Virus Taxonomy ID: 11786 Superkingdom: Viruses Kingdom: not available Genus/species: Gammaretrovirus not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pCNA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MLV_Nucleocapsid: ATVVSGQKQDRQGGERRRSQ LDRDQCAYCKEKGHWAKDCP KKPRGPRGPRPQTSL
tRNApro: GGCUCGUUGGUCUAGGGGUA UGAUUCUCGCUUAGGGUGCG AGAGGUCCCGGGUUCAAAUC CCGGACGAGCC

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	33
1H chemical shifts	250

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	RNA (71-MER)	2
3	ZINC ION	3

Entities:

Entity 1, entity_1 55 residues - 6264.138 Da.

1

ALA

THR

VAL

SER

GLY

GLN

LYS

GLN

ASP

2

ARG

GLN

GLY

GLU

ARG

SER

GLN

3

LEU

ASP

ARG

ASP

GLN

CYS

ALA

TYR

CYS

LYS

4

GLU

LYS

GLY

HIS

TRP

ALA

LYS

ASP

CYS

PRO

5

LYS

PRO

ARG

GLY

PRO

ARG

GLY

PRO

ARG

6

PRO

GLN

THR

SER

LEU

Entity 2, RNA (71-MER) 71 residues - Formula weight is not available

1

G

C

U

C

G

U

G

2

U

C

U

A

G

U

A

3

U

G

A

U

C

U

C

G

C

4

U

A

G

U

G

C

G

5

A

G

A

G

U

C

G

6

G

U

C

A

U

C

7

C

G

A

C

G

A

G

C

8

C

Entity 3, ZINC ION - Zn - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	isotropic	sample_conditions_1
2D 1H-15N HSQC	not available	isotropic	sample_conditions_1
2D 1H-13C HSQC	not available	isotropic	sample_conditions_1
2D 1H-13C HSQC	not available	isotropic	sample_conditions_1
2D 1H-13C HSQC	not available	isotropic	sample_conditions_1
2D 1H-13C HSQC	not available	isotropic	sample_conditions_1
2D NOESY	not available	isotropic	sample_conditions_1
2D NOESY	not available	isotropic	sample_conditions_1
2D NOESY	not available	isotropic	sample_conditions_1
2D NOESY	not available	isotropic	sample_conditions_1

BMRB	25052 25100
PDB	1A6B 1U6P 1WWD 1WWE 1WWF 1WWG 2MQV 2MS0 2MS1
GB	AAB59942 AAB64159 AAC82566 AAC82568 AAL69908
PRF	0711245A
REF	NP_057933 NP_057934 NP_955586
SP	P03332 P03355 Q8UN02
AlphaFold	P03332 P03355 Q8UN02

BMRB Entry 25101

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: