BMRB Entry 25035
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25035
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Citation: Pineda-Sanabria, Sandra; Julien, Olivier; Sykes, Brian. "Versatile cardiac troponin chimera for muscle protein structural biology and drug discovery." ACS Chem. Biol. 9, 2121-2130 (2014).
PubMed: 25010113
Assembly members:
cChimeraX, polymer, 141 residues, 15937 Da.
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet3a
Entity Sequences (FASTA):
cChimeraX: MHHHHHHGGLVPRGSMDDIY
KAAVEQLTEEQKNEFKAAFD
IFVLGAEDGSISTKELGKVM
RMLGQNPTPEELQEMIDEVD
EDGSGTVDFDEFLVMMVRCM
KDDSENLYFQGRRVRISADA
MMQALLGARAKESLDLRAHL
K
- assigned_chemical_shifts
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
| Data type | Count |
| 13C chemical shifts | 188 |
| 1H chemical shifts | 101 |
| 15N chemical shifts | 101 |
| heteronuclear NOE values | 193 |
| T1 relaxation values | 194 |
| T2 relaxation values | 201 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cChimeraX | 1 |
| 2 | CALCIUM ION | 2 |
Entities:
Entity 1, cChimeraX 141 residues - 15937 Da.
Residues -15 to -7 correspond to the His tag. Residues -6 to -1 correspond to the thrombin cleavage site. Residues 1 to 89 correspond to residues 1 to 89 of cNTnC. Residues 90 to 96 correspond to the thrombin cleavage site. Residues 144 to 173 correspond to 144 to 173 of cTnI.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | GLY | GLY | LEU | ||||
| 2 | VAL | PRO | ARG | GLY | SER | MET | ASP | ASP | ILE | TYR | ||||
| 3 | LYS | ALA | ALA | VAL | GLU | GLN | LEU | THR | GLU | GLU | ||||
| 4 | GLN | LYS | ASN | GLU | PHE | LYS | ALA | ALA | PHE | ASP | ||||
| 5 | ILE | PHE | VAL | LEU | GLY | ALA | GLU | ASP | GLY | SER | ||||
| 6 | ILE | SER | THR | LYS | GLU | LEU | GLY | LYS | VAL | MET | ||||
| 7 | ARG | MET | LEU | GLY | GLN | ASN | PRO | THR | PRO | GLU | ||||
| 8 | GLU | LEU | GLN | GLU | MET | ILE | ASP | GLU | VAL | ASP | ||||
| 9 | GLU | ASP | GLY | SER | GLY | THR | VAL | ASP | PHE | ASP | ||||
| 10 | GLU | PHE | LEU | VAL | MET | MET | VAL | ARG | CYS | MET | ||||
| 11 | LYS | ASP | ASP | SER | GLU | ASN | LEU | TYR | PHE | GLN | ||||
| 12 | GLY | ARG | ARG | VAL | ARG | ILE | SER | ALA | ASP | ALA | ||||
| 13 | MET | MET | GLN | ALA | LEU | LEU | GLY | ALA | ARG | ALA | ||||
| 14 | LYS | GLU | SER | LEU | ASP | LEU | ARG | ALA | HIS | LEU | ||||
| 15 | LYS |
Entity 2, CALCIUM ION - Ca - 40.078 Da.
| 1 | CA |
Related Database Links:
| BMRB | 15385 15388 15400 15427 16190 16752 17103 19789 25120 25495 25797 |
| PDB | 1AJ4 1AP4 1DTL 1J1D 1J1E 1LA0 1LXF 1MXL 1SPY 1WRK 1WRL 2CTN 2JT0 2JT3 2JT8 2JTZ 2KFX 2KGB 2KRD 2L1R 2MKP 2MZP 3SD6 3SWB 4GJE 4GJF |
| DBJ | BAA02369 BAG36483 |
| EMBL | CAA30736 CAG46663 CAG46683 |
| GB | AAA36772 AAA37492 AAA37493 AAA48654 AAB91994 |
| PIR | S07450 TPHUCC |
| PRF | 1403394A 1510257A 750650A |
| REF | NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793 |
| SP | P02591 P05936 P09860 P19123 P63315 |
| TPG | DAA16908 |
| AlphaFold | P02591 P05936 P09860 P19123 P63315 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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