BMRB Entry 18833

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PDB ID: 2m0z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18833
MolProbity Validation Chart

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Title:
cis form of a photoswitchable PDZ domain crosslinked with an azobenzene derivative
Deposition date:
2012-11-09
Original release date:
2013-07-01
Authors:
Walser, Reto; Zerbe, Oliver; Hamm, Peter
Citation:

Citation: Buchli, Brigitte; Waldauer, Steven; Walser, Reto; Donten, Mateusz; Pfister, Rolf; Blochliger, Nicolas; Steiner, Sandra; Caflisch, Amedeo; Zerbe, Oliver; Hamm, Peter. "Kinetic response of a photoperturbed allosteric protein."  Proc. Natl. Acad. Sci. U.S.A. 110, 11725-11730 (2013).
PubMed: 23818626

Assembly members:

Assembly members:
PDZ, polymer, 97 residues, 10067.491 Da.
3,3'-(E)-diazene-1,2-diylbis{6-[(chloroacetyl)amino]benzenesulfonic acid}, non-polymer, 525.340 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ: GPKPGDIFEVELAKNDNSLG ICVTGGVNTSVRHGGIYVKA VIPQGAAESDGRIHKGDRVL AVNGVSLEGATHKQAVCTLR NTGQVVHLLLEKGQSPT

Data sets:
Data typeCount
13C chemical shifts394
15N chemical shifts90
1H chemical shifts639

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ1
2entity_33B2

Entities:

Entity 1, PDZ 97 residues - 10067.491 Da.

1   GLYPROLYSPROGLYASPILEPHEGLUVAL
2   GLULEUALALYSASNASPASNSERLEUGLY
3   ILECYSVALTHRGLYGLYVALASNTHRSER
4   VALARGHISGLYGLYILETYRVALLYSALA
5   VALILEPROGLNGLYALAALAGLUSERASP
6   GLYARGILEHISLYSGLYASPARGVALLEU
7   ALAVALASNGLYVALSERLEUGLUGLYALA
8   THRHISLYSGLNALAVALCYSTHRLEUARG
9   ASNTHRGLYGLNVALVALHISLEULEULEU
10   GLULYSGLYGLNSERPROTHR

Entity 2, entity_33B - C16 H14 Cl2 N4 O8 S2 - 525.340 Da.

1   33B

Related Database Links:

BMRB 18834 4123 4124 4516
PDB 1D5G 2M0Z 2M10 3LNX 3LNY 3PDZ

Download HSQC peak lists in one of the following formats:
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