BMRB Entry 4124
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4124
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Citation: Ekiel, Irena; Banville, Denis; Shen, Shi Hsiang; Slon-Usakiewicz, Jacek; Koshy, Alex; Gehring, Kalle. "Main-chain Signal Assignment for the PDZ2Domain from Human
Protein Tyrosine Phosphatase hPTP1E and its Complex with a C-terminal Peptide
from the Fas Receptor" J. Biomol. NMR 12, 455-456 (1998).
Assembly members:
second PDZ domain from hPTP1E, polymer, 96 residues, 10006 Da.
C-terminal peptide from the Fas receptor, polymer, 15 residues, 1752 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
second PDZ domain from hPTP1E: PKPGDIFEVELAKNDNSLGI
SVTGGVNTSVRHGGIYVKAV
IPQGAAESDGRIHKGDRVLA
VNGVSLEGATHKQAVETLRN
TGQVVHLLLEKGQSPT
C-terminal peptide from the Fas receptor: DSENSNFRNEIQSLV
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDZ2 | 1 |
| 2 | Fas_Cterm15 | 2 |
Entities:
Entity 1, PDZ2 96 residues - 10006 Da.
| 1 | PRO | LYS | PRO | GLY | ASP | ILE | PHE | GLU | VAL | GLU | ||||
| 2 | LEU | ALA | LYS | ASN | ASP | ASN | SER | LEU | GLY | ILE | ||||
| 3 | SER | VAL | THR | GLY | GLY | VAL | ASN | THR | SER | VAL | ||||
| 4 | ARG | HIS | GLY | GLY | ILE | TYR | VAL | LYS | ALA | VAL | ||||
| 5 | ILE | PRO | GLN | GLY | ALA | ALA | GLU | SER | ASP | GLY | ||||
| 6 | ARG | ILE | HIS | LYS | GLY | ASP | ARG | VAL | LEU | ALA | ||||
| 7 | VAL | ASN | GLY | VAL | SER | LEU | GLU | GLY | ALA | THR | ||||
| 8 | HIS | LYS | GLN | ALA | VAL | GLU | THR | LEU | ARG | ASN | ||||
| 9 | THR | GLY | GLN | VAL | VAL | HIS | LEU | LEU | LEU | GLU | ||||
| 10 | LYS | GLY | GLN | SER | PRO | THR |
Entity 2, Fas_Cterm15 15 residues - 1752 Da.
| 1 | ASP | SER | GLU | ASN | SER | ASN | PHE | ARG | ASN | GLU | ||||
| 2 | ILE | GLN | SER | LEU | VAL |
Samples:
sample_one: hPTP1E-Fas receptor complex, [U-15N; U-13C], 3 mM; sodium phosphate buffer 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%
sample_conditions_one: pH: 6.9; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DRX500 500 MHz
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CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks