BMRB Entry 18770

Name: TatA T22P
Published: 2013-03-18

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PDB ID: 2lzr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18770
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TatA T22P

Deposition date:

2012-10-08

Original release date:

2013-03-18

Authors:

Rodriguez, Fernanda; Berks, Ben; Schnell, Jason

Citation:

Citation: Rodriguez, Fernanda; Rouse, Sarah; Tait, Claudia; Harmer, Jeffrey; De Riso, Antonio; Timmel, Christiane; Sansom, Mark; Berks, Ben; Schnell, Jason. "Structural model for the protein-translocating element of the twin-arginine transport system." Proc. Natl. Acad. Sci. U.S.A. 110, E1092-E1101 (2013).
PubMed: 23471988

Assembly members:

Assembly members:
TatA_T22P, polymer, 49 residues, 5177.260 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24 TatAd40 T22P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TatA_T22P: XGGISIWQLLIIAVIVVLLF GPKKLGSIGSDLGASIKGFK KAMSDDEPK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	144
15N chemical shifts	48
1H chemical shifts	339

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TatA_T22P	1

Entities:

Entity 1, TatA_T22P 49 residues - 5177.260 Da.

1

FME

GLY

ILE

SER

ILE

TRP

GLN

LEU

2

ILE

ALA

VAL

ILE

VAL

LEU

PHE

3

GLY

PRO

LYS

LEU

GLY

SER

ILE

GLY

SER

4

ASP

LEU

GLY

ALA

SER

ILE

LYS

GLY

PHE

LYS

5

LYS

ALA

MET

SER

ASP

GLU

PRO

LYS

Samples:

N: TatA_T22P, [U-100% 15N], 0.5 mM; DPC 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

NC: TatA_T22P, [U-100% 13C; U-100% 15N], 0.5 mM; DPC 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

NC_deutDPC: TatA_T22P, [U-100% 13C; U-100% 15N], 0.5 mM; DPC, [U-100% 2H], 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCA	NC	isotropic	sample_conditions_1
3D HNCO	NC	isotropic	sample_conditions_1
3D 1H-15N NOESY	N	isotropic	sample_conditions_1
3D 1H-15N TOCSY	N	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	NC_deutDPC	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	NC_deutDPC	isotropic	sample_conditions_1
3D HNCO	NC	anisotropic	sample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Homebuilt OMEGA 950 MHz
Homebuilt OMEGA 600 MHz
Homebuilt OMEGA 750 MHz
Bruker AVII 500 MHz

BMRB	18771 19714 19881
PDB	2LZR 2LZS 2MN6 2MN7
DBJ	BAB38189 BAE77465 BAG79648 BAI27909 BAI33032
EMBL	CAA06724 CAP78301 CAQ34195 CAQ91106 CAR00812
GB	AAA67633 AAC19240 AAC76839 AAG59032 AAN45349
PIR	D86071
REF	NP_312793 NP_418280 NP_709642 WP_000508966 WP_000508967
SP	P69428 P69429 P69430 P69431
AlphaFold	P69428 P69429 P69430 P69431