BMRB Entry 18770

Title:
TatA T22P
Deposition date:
2012-10-08
Original release date:
2013-03-18
Authors:
Rodriguez, Fernanda; Berks, Ben; Schnell, Jason
Citation:

Citation: Rodriguez, Fernanda; Rouse, Sarah; Tait, Claudia; Harmer, Jeffrey; De Riso, Antonio; Timmel, Christiane; Sansom, Mark; Berks, Ben; Schnell, Jason. "Structural model for the protein-translocating element of the twin-arginine transport system."  Proc. Natl. Acad. Sci. U.S.A. 110, E1092-E1101 (2013).
PubMed: 23471988

Assembly members:

Assembly members:
TatA_T22P, polymer, 49 residues, 5177.260 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24 TatAd40 T22P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TatA_T22P: XGGISIWQLLIIAVIVVLLF GPKKLGSIGSDLGASIKGFK KAMSDDEPK

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts48
1H chemical shifts339

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TatA_T22P1

Entities:

Entity 1, TatA_T22P 49 residues - 5177.260 Da.

1   FMEGLYGLYILESERILETRPGLNLEULEU
2   ILEILEALAVALILEVALVALLEULEUPHE
3   GLYPROLYSLYSLEUGLYSERILEGLYSER
4   ASPLEUGLYALASERILELYSGLYPHELYS
5   LYSALAMETSERASPASPGLUPROLYS

Samples:

N: TatA_T22P, [U-100% 15N], 0.5 mM; DPC 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

NC: TatA_T22P, [U-100% 13C; U-100% 15N], 0.5 mM; DPC 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

NC_deutDPC: TatA_T22P, [U-100% 13C; U-100% 15N], 0.5 mM; DPC, [U-100% 2H], 240 mM; H2O 95%; D2O 5%; sodium phosphate 0.05 M

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D HNCANCisotropicsample_conditions_1
3D HNCONCisotropicsample_conditions_1
3D 1H-15N NOESYNisotropicsample_conditions_1
3D 1H-15N TOCSYNisotropicsample_conditions_1
3D 1H-13C NOESY aliphaticNC_deutDPCisotropicsample_conditions_1
3D 1H-13C NOESY aromaticNC_deutDPCisotropicsample_conditions_1
3D HNCONCanisotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Homebuilt OMEGA 950 MHz
  • Homebuilt OMEGA 600 MHz
  • Homebuilt OMEGA 750 MHz
  • Bruker AVII 500 MHz

Related Database Links:

BMRB 18771 19714 19881
PDB
DBJ BAB38189 BAE77465 BAG79648 BAI27909 BAI33032
EMBL CAA06724 CAP78301 CAQ34195 CAQ91106 CAR00812
GB AAA67633 AAC19240 AAC76839 AAG59032 AAN45349
PIR D86071
REF NP_312793 NP_418280 NP_709642 WP_000508966 WP_000508967
SP P69428 P69429 P69430 P69431
AlphaFold P69428 P69429 P69430 P69431

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks