BMRB Entry 18698

Name: Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A
Published: 2012-10-29

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PDB ID: 2lxu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18698
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A

Deposition date:

2012-08-31

Original release date:

2012-10-29

Authors:

Ramelot, Theresa; Yang, Yunhuang; Pederson, Kari; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Prestegard, James; Montelione, Gaetano; Kennedy, Michael

Citation:

Citation: Ramelot, Theresa; Yang, Yunhuang; Pederson, Kari; Shastry, Ritu; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Prestegard, James; Montelione, Gaetano; Kennedy, Michael. "Solution NMR Structure of the eukaryotic RNA recognition motif, RRM1, from the heterogeneous nuclear ribonucleoprotein H from Homo sapiens, Northeast Structural Genomics Consortium (NESG) Target HR8614A" To be published ., .-..

Assembly members:

Assembly members:
HR8614A, polymer, 108 residues, 12381.880 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15Avi6HT_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR8614A: SHMGGEGFVVKVRGLPWSCS ADEVQRFFSDCKIQNGAQGI RFIYTREGRPSGEAFVELES EDEVKLALKKDRETMGHRYV EVFKSNNVEMDWVLKHTGPN SPDTANDG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
conformer_family_coord_set
representative_conformer
spectral_peak_list

Data type	Count
13C chemical shifts	466
15N chemical shifts	113
1H chemical shifts	723

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HR8614A	1

Entities:

Entity 1, HR8614A 108 residues - 12381.880 Da.

1

SER

HIS

MET

GLY

GLU

GLY

PHE

VAL

2

LYS

VAL

ARG

GLY

LEU

PRO

TRP

SER

CYS

SER

3

ALA

ASP

GLU

VAL

GLN

ARG

PHE

SER

ASP

4

CYS

LYS

ILE

GLN

ASN

GLY

ALA

GLN

GLY

ILE

5

ARG

PHE

ILE

TYR

THR

ARG

GLU

GLY

ARG

PRO

6

SER

GLY

GLU

ALA

PHE

VAL

GLU

LEU

GLU

SER

7

GLU

ASP

GLU

VAL

LYS

LEU

ALA

LEU

LYS

8

ASP

ARG

GLU

THR

MET

GLY

HIS

ARG

TYR

VAL

9

GLU

VAL

PHE

LYS

SER

ASN

VAL

GLU

MET

10

ASP

TRP

VAL

LEU

LYS

HIS

THR

GLY

PRO

ASN

11

SER

PRO

ASP

THR

ALA

ASN

ASP

GLY

Samples:

HR8614A.007_NC_sample: HR8614A.007, [U-100% 13C; U-100% 15N], 1.0 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM

HR8614A.009_NC5_sample: HR8614A.008, [biosynthetically directed-5% 13C; U-100% 15N], 0.8 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM

HR8614A.007_NC_D2O_sample: HR8614A.007, [U-100% 13C; U-100% 15N], 1.0 mM; MES 20 mM; sodium chloride 100 mM; calcium chloride 5 mM; DTT 10 mM; DSS 50 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	HR8614A.007_NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D HNCO	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D CBCA(CO)NH	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D HNCACB	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D 1H-13C arom NOESY	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D 1H-15N NOESY	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D C(CO)NH	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D HBHA(CO)NH	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D HCCH-TOCSY	HR8614A.007_NC_sample	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	HR8614A.007_NC_D2O_sample	isotropic	sample_conditions_1
3D NUS 1H-13C NOESY aliphatic	HR8614A.007_NC_D2O_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2only	HR8614A.007_NC_sample	isotropic	sample_conditions_1
4D 13C-13C-HMQC-NOESY-HMQC	HR8614A.007_NC_D2O_sample	isotropic	sample_conditions_1
1D T1 Nhsqc array	HR8614A.007_NC_sample	isotropic	sample_conditions_1
1D T2 Nhsqc array	HR8614A.007_NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC	HR8614A.009_NC5_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	HR8614A.009_NC5_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC arom CT	HR8614A.007_NC_sample	isotropic	sample_conditions_1
2D 1H-13C HSQC arom noCT	HR8614A.007_NC_sample	isotropic	sample_conditions_1
2D 1H-15N HSQC_His	HR8614A.009_NC5_sample	isotropic	sample_conditions_1
2D 1H-15N heteronuclear NOE interleaved	HR8614A.009_NC5_sample	isotropic	sample_conditions_1

UNP	P31943
HUGO	HNRNPH1
BMRB	15212
PDB	2LXU
GB	AAH99792 EDM04272 EDM04274
AlphaFold	Q6IBM4

BMRB Entry 18698

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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