BMRB Entry 15212

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15212

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Title:
1H, 13C, and 15N Chemical Shift Assignments for the first Nterminal domain, RRM1, of hnRNP H
Deposition date:
2007-04-13
Original release date:
2008-01-28
Authors:
CABAL, Stephanie; Guittet, Eric
Citation:

Citation: Cabal, Stephanie; van Heijenoort, Carine; Guittet, Eric. "1H, 13C and 15N Resonance assignments of the first N-terminal RNA recognition motif (RRM) of the human heterogeneous nuclear ribonucleoprotein H (hnRNP H)"  Biomol. NMR Assignments 1, 221-223 (2007).
PubMed: 19636870

Assembly members:

Assembly members:
RRM1_hnRNPH, polymer, 104 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM1_hnRNPH: MMLGTEGGEGFVVKVRGLPW SCSADEVQRFFSDCKIQNGA QGIRFIYTREGRPSGEAFVE LESEDEVKLALKKDRETMGH RYVEVFKSNNVEMDWVLKHT GPNS

Data sets:
Data typeCount
13C chemical shifts385
15N chemical shifts101
1H chemical shifts628

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1domain 11

Entities:

Entity 1, domain 1 104 residues - Formula weight is not available

1   METMETLEUGLYTHRGLUGLYGLYGLUGLY
2   PHEVALVALLYSVALARGGLYLEUPROTRP
3   SERCYSSERALAASPGLUVALGLNARGPHE
4   PHESERASPCYSLYSILEGLNASNGLYALA
5   GLNGLYILEARGPHEILETYRTHRARGGLU
6   GLYARGPROSERGLYGLUALAPHEVALGLU
7   LEUGLUSERGLUASPGLUVALLYSLEUALA
8   LEULYSLYSASPARGGLUTHRMETGLYHIS
9   ARGTYRVALGLUVALPHELYSSERASNASN
10   VALGLUMETASPTRPVALLEULYSHISTHR
11   GLYPROASNSER

Samples:

sample_1: RRM1 hnRNPH, [U-90% 13C; U-98% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 1 mM; D2O 10%

sample_2: RRM1 hnRNPH, [U-98% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 1 mM; D2O 10%

sample_3: RRM1 hnRNPH, [U-90% 13C; U-95% 15N], 0.8 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 1 mM; D2O 100%

sample_conditions_1: ionic strength: 1 M; pH: 2.2; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18698
PDB
GB AAH99792 EDM04272 EDM04274

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks