BMRB Entry 18671

Assembly:

Entities:

Entity 1, Get5 81 residues - 9170.889 Da.

Entity 2, Sgt2_1 74 residues - 7889.790 Da.

Samples:

sample_1: Get5 1 mM; Sgt2, [U-100% 13C; U-100% 15N], 2 mM; Bis-Tris 10 mM; L-arginine 50 mM; L-glutamic acid 50 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_2: Get5 1 mM; Sgt2, [U-100% 13C; U-100% 15N], 2 mM; Bis-Tris 10 mM; L-arginine 50 mM; L-glutamic acid 50 mM; sodium azide 0.02%; polyacrylamide 3.5%; H2O 90%; D2O 10%

sample_3: Get5, [U-100% 13C; U-100% 15N], 1 mM; Sgt2 2 mM; Bis-Tris 10 mM; L-arginine 50 mM; L-glutamic acid 50 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_4: Get5, [U-100% 13C; U-100% 15N], 1 mM; Sgt2 2 mM; Bis-Tris 10 mM; L-arginine 50 mM; L-glutamic acid 50 mM; sodium azide 0.02%; polyacrylamide 3.5%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 mM; pH: 6.1; pressure: 1 atm; temperature: 310 K

Experiments:

Software:

VNMRJ, Varian - collection

Analysis, CCPN - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS+, Cornilescu, Delaglio and Bax - data analysis

ARIA v2.3, Linge, O'Donoghue and Nilges - refinement, structure solution

HADDOCK v2.1, Alexandre Bonvin - refinement, structure solution

TOPSPIN, Bruker Biospin - collection

PALES, Zweckstetter and Bax - data analysis

NMR spectrometers:

• Varian INOVA 600 MHz
• Bruker Avance 800 MHz