BMRB Entry 18649

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18649
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
Transmembrane domain of Amyloid precursor protein WT
Deposition date:
2012-08-07
Original release date:
2012-09-07
Authors:
Chen, Wen; Wang, Chunyu
Citation:

Citation: Chen, Wen; Gamache, Eric; Rosenman, David; Xie, Jian; Lopez, Maria; Li, Yue-Ming; Wang, Chunyu. "Familial Alzheimer's mutations within APPTM increase A42 production by enhancing accessibility of -cleavage site."  Nat. Commun. 5, 3037-3037 (2014).
PubMed: 24390130

Assembly members:

Assembly members:
wild type monomer, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PetM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
wild type monomer: GAMAKGAIIGLMVGGVVIAT VIVITLVMLKKK

Data sets:
Data typeCount
13C chemical shifts144
15N chemical shifts30
1H chemical shifts252

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1wild type chain, 11
2wild type chain, 21

Entities:

Entity 1, wild type chain, 1 32 residues - Formula weight is not available

1   GLYALAMETALALYSGLYALAILEILEGLY
2   LEUMETVALGLYGLYVALVALILEALATHR
3   VALILEVALILETHRLEUVALMETLEULYS
4   LYSLYS

Samples:

apptm_wt_sample: APPTM dimer, [U-100% 13C; U-100% 15N], mM; sodium phosphate 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 7.2; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCapptm_wt_sampleisotropicsample_conditions_1
2D 1H-13C HSQCapptm_wt_sampleisotropicsample_conditions_1
3D HNCACBapptm_wt_sampleisotropicsample_conditions_1
3D HBHA(CO)NHapptm_wt_sampleisotropicsample_conditions_1
3D HNCOapptm_wt_sampleisotropicsample_conditions_1
3D HCCH-TOCSYapptm_wt_sampleisotropicsample_conditions_1
3D HNHAapptm_wt_sampleisotropicsample_conditions_1
3D H(CCO)NHapptm_wt_sampleisotropicsample_conditions_1

Software:

SPARKY, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15775 18080
PDB
DBJ BAA22264 BAA24230 BAA84580 BAB71958 BAC34997
EMBL CAA30050 CAA30488 CAA31830 CAA39589 CAA39590
GB AAA35540 AAA36829 AAA37139 AAA51722 AAA51726
PIR A60045 D60045 E60045 G60045 JH0773
PRF 1303338A 1403400A 1507304A 1507304B 1507304C
REF NP_000475 NP_001005698 NP_001006601 NP_001013036 NP_001070264
SP P05067 P08592 P12023 P53601 P79307
TPG DAA33655
AlphaFold P05067 P08592 P12023 P53601 P79307

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks