BMRB Entry 18485

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PDB ID: 2ltk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18485
MolProbity Validation Chart

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Title:
Solution structure of a monomeric truncated mutant of Trypanosoma brucei 1-C-Grx1
Deposition date:
2012-05-29
Original release date:
2013-04-02
Authors:
Bellanda, Massimo; Pavan, Carlo; Gesiot, Lorenzo; Comini, Marcelo; Manta, Bruno; Mammi, Stefano; Sturlese, Mattia
Citation:

Citation: Manta, Bruno; Pavan, Carlo; Sturlese, Mattia; Medeiros, Andrea; Crispo, Martina; Berndt, Carsten; Krauth-Siegel, R.; Bellanda, Massimo; Comini, Marcelo. "Iron-sulfur cluster binding by mitochondrial monothiol glutaredoxin-1 of Trypanosoma brucei: molecular basis of iron-sulfur cluster coordination and relevance for parasite infectivity"  Antioxid. Redox Signal. 19, 665-682 (2013).
PubMed: 23259530

Assembly members:

Assembly members:
1-C-Grx1, polymer, 110 residues, 12325.543 Da.

Natural source:

Natural source:   Common Name: kinetoplastids   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PETM-20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
1-C-Grx1: GAMVKKDIDDTIKSEDVVTF IKGLPEAPMCAYSKRMIDVL EALGLEYTSFDVLAHPVVRS YVKEVSEWPTIPQLFIKAEF VGGLDIVTKMLESGDLKKML RDKGITCRDL

Data sets:
Data typeCount
13C chemical shifts496
15N chemical shifts103
1H chemical shifts816

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tb 1-C-Grx1 monomer1

Entities:

Entity 1, Tb 1-C-Grx1 monomer 110 residues - 12325.543 Da.

1   GLYALAMETVALLYSLYSASPILEASPASP
2   THRILELYSSERGLUASPVALVALTHRPHE
3   ILELYSGLYLEUPROGLUALAPROMETCYS
4   ALATYRSERLYSARGMETILEASPVALLEU
5   GLUALALEUGLYLEUGLUTYRTHRSERPHE
6   ASPVALLEUALAHISPROVALVALARGSER
7   TYRVALLYSGLUVALSERGLUTRPPROTHR
8   ILEPROGLNLEUPHEILELYSALAGLUPHE
9   VALGLYGLYLEUASPILEVALTHRLYSMET
10   LEUGLUSERGLYASPLEULYSLYSMETLEU
11   ARGASPLYSGLYILETHRCYSARGASPLEU

Related Database Links:

BMRB 19736
PDB 2LTK
EMBL CAF02300 CBH14109
GB EAN76448
REF XP_011776380 XP_803662

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