BMRB Entry 19736

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19736
MolProbity Validation Chart

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Title:
1H, 13C, and 15N Chemical Shift Assignments of a the mature form of Trypanosoma brucei 1-C-Grx1
Deposition date:
2014-01-15
Original release date:
2014-11-07
Authors:
Sturlese, Mattia; Lelli, Moreno; Bellanda, Massimo; Manta, Bruno; Comini, Marcelo
Citation:

Citation: Sturlese, Mattia; Lelli, Moreno; Manta, Bruno; Mammi, Stefano; Comini, Marcelo; Bellanda, Massimo. "1H, 13C and 15N resonance assignment of the mature form of monothiol glutaredoxin 1 from the pathogen Trypanosoma brucei"  Biomol. NMR Assignments ., .-. (2014).
PubMed: 24830542

Assembly members:

Assembly members:
1-C-Grx1, polymer, 145 residues, 16063.5 Da.

Natural source:

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 5691   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET Trx-1b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
1-C-Grx1: GAMSTSGIGGDVRDIEETHP DFQPRLVSADLAEDEIAMVK KDIDDTIKSEDVVTFIKGLP EAPMCAYSKRMIDVLEALGL EYTSFDVLAHPVVRSYVKEV SEWPTIPQLFIKAEFVGGLD IVTKMLESGDLKKMLRDKGI TCRDL

Data sets:
Data typeCount
13C chemical shifts594
15N chemical shifts132
1H chemical shifts869

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11-C-Grx11

Entities:

Entity 1, 1-C-Grx1 145 residues - 16063.5 Da.

Residues 1-3 represent the residual cleavage site sequence.

1   GLYALAMETSERTHRSERGLYILEGLYGLY
2   ASPVALARGASPILEGLUGLUTHRHISPRO
3   ASPPHEGLNPROARGLEUVALSERALAASP
4   LEUALAGLUASPGLUILEALAMETVALLYS
5   LYSASPILEASPASPTHRILELYSSERGLU
6   ASPVALVALTHRPHEILELYSGLYLEUPRO
7   GLUALAPROMETCYSALATYRSERLYSARG
8   METILEASPVALLEUGLUALALEUGLYLEU
9   GLUTYRTHRSERPHEASPVALLEUALAHIS
10   PROVALVALARGSERTYRVALLYSGLUVAL
11   SERGLUTRPPROTHRILEPROGLNLEUPHE
12   ILELYSALAGLUPHEVALGLYGLYLEUASP
13   ILEVALTHRLYSMETLEUGLUSERGLYASP
14   LEULYSLYSMETLEUARGASPLYSGLYILE
15   THRCYSARGASPLEU

Samples:

sample_1: 1-C-Grx1, [U-13C; U-15N], 1.8 mM; DTT 5 mM; sodium phosphate 50 mM; sodium chloride 150 mM; sodium azide 0.05 % w/v; EDTA 1 mM; PMSF 0.2 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

CARA v1.9.0, Fred Damberger, Rochus Keller - chemical shift assignment, peak picking

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 1000 MHz
  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

EMBL AJ619696.1 CAF02300 CBH14109
UNIPROT Q2UZM9
NCBI CAF02300
BMRB 18485
PDB
GB EAN76448
REF XP_011776380 XP_803662
AlphaFold Q2UZM9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks