BMRB Entry 18342

Name: Backbone and sidechain assignments for Get5_UBL domain
Published: 2012-11-15

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PDB ID: 4asw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18342
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and sidechain assignments for Get5_UBL domain

Deposition date:

2012-03-21

Original release date:

2012-11-15

Authors:

Simon, Aline; Simpson, Peter; Isaacson, Rivka

Citation:

Citation: Simon, Aline; Simpson, Peter; Hawthorne, William; Hale, Lisa; Goldstone, Rachael; Isaacson, Rivka. "1H, 13C and 15N assignments of Sgt2N-terminal dimerisation domain and its binding partner, Get5 Ubiquitin-like domain." Biomol. NMR Assignments 7, 271-274 (2013).
PubMed: 23001946

Assembly members:

Assembly members:
Get5_ubl, polymer, 97 residues, 10963.5 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Get5_ubl: AHHHHHHVDDDDKMDNAAVH LTLKKIQAPKFSIEHDFSPS DTILQIKQHLISEEKASHIS EIKLLLKGKVLHDNLFLSDL KVTPANSTITVMIKPNP

Data sets:

assigned_chemical_shifts
- assignments_get5_ubl_minus1

Data type	Count
13C chemical shifts	383
15N chemical shifts	82
1H chemical shifts	601

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Get5_ubl	1

Entities:

Entity 1, Get5_ubl 97 residues - 10963.5 Da.

1

ALA

HIS

VAL

ASP

2

ASP

LYS

MET

ASP

ASN

ALA

VAL

HIS

3

LEU

THR

LEU

LYS

ILE

GLN

ALA

PRO

LYS

4

PHE

SER

ILE

GLU

HIS

ASP

PHE

SER

PRO

SER

5

ASP

THR

ILE

LEU

GLN

ILE

LYS

GLN

HIS

LEU

6

ILE

SER

GLU

LYS

ALA

SER

HIS

ILE

SER

7

GLU

ILE

LYS

LEU

LYS

GLY

LYS

VAL

8

LEU

HIS

ASP

ASN

LEU

PHE

LEU

SER

ASP

LEU

9

LYS

VAL

THR

PRO

ALA

ASN

SER

THR

ILE

THR

10

VAL

MET

ILE

LYS

PRO

ASN

PRO

Samples:

sample_1: Get5_ubl, [U-98% 13C; U-98% 15N], 700 uM; MES 100 mM; potassium chloride 150 mM; TCEP 0.5 mM; H2O 90%; D2O 10%

sample_2: Get5_ubl, [U-98% 13C; U-98% 15N], 700 uM; MES 100 mM; potassium chloride 150 mM; TCEP 0.5 mM; D2O 100%

sample_conditions_1: ionic strength: 0.25 M; pH: 6; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_2	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz