BMRB Entry 18234
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PDB ID: 2lp4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18234
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Mo, Guoya; Zhou, Hongjun; Kamamura, Tetsuya; dahlquist, Frederick. "Solution structure of P1-CheY/P2 complex in bacterial chemotaxis" Biochemistry ., .-..
Assembly members:
CheA_P1P2, polymer, 225 residues, 24991.010 Da.
CheY, polymer, 128 residues, 13981.248 Da.
Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE12
Entity Sequences (FASTA):
CheA_P1P2: MSMDISDFYQTFFDEADELL
ADMEQHLLDLVPESPDAEQL
NAIFRAAHSIKGGAGTFGFT
ILQETTHLMENLLDEARRGE
MQLNTDIINLFLETKDIMQE
QLDAYKNSEEPDAASFEYIC
NALRQLALEAKGETPSAVTR
LSVVAKSEPQDEQSRSQSPR
RIILSRLKAGEVDLLEEELG
HLTTLTDVVKGADSLSAILP
GDIAEDDITAVLCFVIEADQ
ITFET
CheY: ADKELKFLVVDDFSTMRRIV
RNLLKELGFNNVEEAEDGVD
ALNKLQAGGYGFVISDWNMP
NMDGLELLKTIRADGAMSAL
PVLMVTAEAKKENIIAAAQA
GASGYVVKPFTAATLEEKLN
KIFEKLGM
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 108 |
| 1H chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CheA_P1P2 | 1 |
| 2 | CheY | 2 |
Entities:
Entity 1, CheA_P1P2 225 residues - 24991.010 Da.
| 1 | MET | SER | MET | ASP | ILE | SER | ASP | PHE | TYR | GLN | ||||
| 2 | THR | PHE | PHE | ASP | GLU | ALA | ASP | GLU | LEU | LEU | ||||
| 3 | ALA | ASP | MET | GLU | GLN | HIS | LEU | LEU | ASP | LEU | ||||
| 4 | VAL | PRO | GLU | SER | PRO | ASP | ALA | GLU | GLN | LEU | ||||
| 5 | ASN | ALA | ILE | PHE | ARG | ALA | ALA | HIS | SER | ILE | ||||
| 6 | LYS | GLY | GLY | ALA | GLY | THR | PHE | GLY | PHE | THR | ||||
| 7 | ILE | LEU | GLN | GLU | THR | THR | HIS | LEU | MET | GLU | ||||
| 8 | ASN | LEU | LEU | ASP | GLU | ALA | ARG | ARG | GLY | GLU | ||||
| 9 | MET | GLN | LEU | ASN | THR | ASP | ILE | ILE | ASN | LEU | ||||
| 10 | PHE | LEU | GLU | THR | LYS | ASP | ILE | MET | GLN | GLU | ||||
| 11 | GLN | LEU | ASP | ALA | TYR | LYS | ASN | SER | GLU | GLU | ||||
| 12 | PRO | ASP | ALA | ALA | SER | PHE | GLU | TYR | ILE | CYS | ||||
| 13 | ASN | ALA | LEU | ARG | GLN | LEU | ALA | LEU | GLU | ALA | ||||
| 14 | LYS | GLY | GLU | THR | PRO | SER | ALA | VAL | THR | ARG | ||||
| 15 | LEU | SER | VAL | VAL | ALA | LYS | SER | GLU | PRO | GLN | ||||
| 16 | ASP | GLU | GLN | SER | ARG | SER | GLN | SER | PRO | ARG | ||||
| 17 | ARG | ILE | ILE | LEU | SER | ARG | LEU | LYS | ALA | GLY | ||||
| 18 | GLU | VAL | ASP | LEU | LEU | GLU | GLU | GLU | LEU | GLY | ||||
| 19 | HIS | LEU | THR | THR | LEU | THR | ASP | VAL | VAL | LYS | ||||
| 20 | GLY | ALA | ASP | SER | LEU | SER | ALA | ILE | LEU | PRO | ||||
| 21 | GLY | ASP | ILE | ALA | GLU | ASP | ASP | ILE | THR | ALA | ||||
| 22 | VAL | LEU | CYS | PHE | VAL | ILE | GLU | ALA | ASP | GLN | ||||
| 23 | ILE | THR | PHE | GLU | THR |
Entity 2, CheY 128 residues - 13981.248 Da.
| 1 | ALA | ASP | LYS | GLU | LEU | LYS | PHE | LEU | VAL | VAL | ||||
| 2 | ASP | ASP | PHE | SER | THR | MET | ARG | ARG | ILE | VAL | ||||
| 3 | ARG | ASN | LEU | LEU | LYS | GLU | LEU | GLY | PHE | ASN | ||||
| 4 | ASN | VAL | GLU | GLU | ALA | GLU | ASP | GLY | VAL | ASP | ||||
| 5 | ALA | LEU | ASN | LYS | LEU | GLN | ALA | GLY | GLY | TYR | ||||
| 6 | GLY | PHE | VAL | ILE | SER | ASP | TRP | ASN | MET | PRO | ||||
| 7 | ASN | MET | ASP | GLY | LEU | GLU | LEU | LEU | LYS | THR | ||||
| 8 | ILE | ARG | ALA | ASP | GLY | ALA | MET | SER | ALA | LEU | ||||
| 9 | PRO | VAL | LEU | MET | VAL | THR | ALA | GLU | ALA | LYS | ||||
| 10 | LYS | GLU | ASN | ILE | ILE | ALA | ALA | ALA | GLN | ALA | ||||
| 11 | GLY | ALA | SER | GLY | TYR | VAL | VAL | LYS | PRO | PHE | ||||
| 12 | THR | ALA | ALA | THR | LEU | GLU | GLU | LYS | LEU | ASN | ||||
| 13 | LYS | ILE | PHE | GLU | LYS | LEU | GLY | MET |
Related Database Links:
| PDB | 2LP4 1A0O 1AB5 1AB6 1BDJ 1C4W 1CEY 1CHN 1CYE 1D4Z 1DJM 1E6K 1E6L 1E6M 1EAY 1EHC 1F4V 1FFG 1FFS 1FFW 1FQW 1JBE 1KMI 1MIH 1VLZ 1YMU 1ZDM 2B1J 2CHE 2CHF 2FKA 2FLK 2FLW 2FMF 2FMH 2FMI 2FMK 2ID7 2ID9 2IDM 2PL9 2PMC 3CHY 3F7N 3FFT 3FFW 3FFX 3FGZ 3MYY 3OLV 3OLW 3OLX 3OLY 3OO0 3OO1 3RVJ 3RVK 3RVL 3RVM 3RVN 3RVO 3RVP 3RVQ 3RVR 3RVS 5CHY 6CHY |
| EMBL | CQF39165 CAD05667 CAP76371 CAQ32359 CAQ98822 CAR03242 |
| GB | EIQ53621 AAA23570 AAA23577 AAA27037 AAC74952 AAG56872 |
| REF | WP_042351715 NP_288319 NP_310619 NP_416396 NP_456482 NP_460873 |
| BMRB | 2950 2951 3440 4083 4472 |
| DBJ | BAA15698 BAB36015 BAG77641 BAI25973 BAI30936 |
| PIR | AH0745 |
| SP | P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69 |
| AlphaFold | P0A2D5 P0A2D6 P0AE67 P0AE68 P0AE69 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks