BMRB Entry 18232

Name: Solid-state NMR assignments for mouse alpha-synuclein fibrils
Published: 2012-04-23

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18232

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR assignments for mouse alpha-synuclein fibrils

Deposition date:

2012-01-31

Original release date:

2012-04-23

Authors:

Lv, Guohua; Kumar, Ashutosh; Giller, Karin; Orcellet, Maria; Riedel, Dietmar; Fernandez, Claudio; Becker, Stefan

Citation:

Citation: Lv, Guohua; Kumar, Ashutosh; Giller, Karin; Orcellet, Maria; Riedel, Dietmar; Fernandez, Claudio; Becker, Stefan; Lange, Adam. "Structural Comparison of Mouse and Human -Synuclein Amyloid Fibrils by Solid-State NMR." J. Mol. Biol. 420, 99-111 (2012).
PubMed: 22516611

Assembly members:

Assembly members:
mouse_alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PT-7-7-mouse alpha-synuclein

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mouse_alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAAGKTKEGVLYV GSKTKEGVVHGVTTVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGNIAAATGFVKKDQM GKGEEGYPQEGILEDMPVDP GSEAYEMPSEEGYQDYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	213
15N chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	mouse alpha-synuclein	1

Entities:

Entity 1, mouse alpha-synuclein 140 residues - Formula weight is not available

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
2	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
3	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
4	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
5	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
6	GLY	VAL	THR	THR	VAL	ALA	GLU	LYS	THR	LYS
7	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
8	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	ASN	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	MET
11	GLY	LYS	GLY	GLU	GLU	GLY	TYR	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	GLY	SER	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

Samples:

sample_1: mouse alpha-synuclein, [U-100% 13C; U-100% 15N], 10 mg; TRIS 25 mM

sample_2: mouse alpha-synuclein, [2-Glucose 13C; U-100% 15N], 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_3: mouse alpha-synuclein, [1-Glucose 13C; U-100% 15N], 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_4: mouse alpha-synuclein, mixed 1:1 13C:15N, 10 mg; TRIS 25 mM; DSS 0.1-0.5 mg

sample_conditions_1: pH: 7.5; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D PDSD 20 ms	sample_1	solid	sample_conditions_1
2D PDSD 150 ms	sample_1	solid	sample_conditions_1
2D DREAM	sample_1	solid	sample_conditions_1
2D NCACX PDSD	sample_1	solid	sample_conditions_1
2D NCOCX PDSD	sample_1	solid	sample_conditions_1
3D NCACX PDSD	sample_1	solid	sample_conditions_1
3D NCOCX PDSD	sample_1	solid	sample_conditions_1
2D NCA	sample_1	solid	sample_conditions_1
2D NHHC	sample_1	solid	sample_conditions_1
2D PDSD 500 ms	sample_2	solid	sample_conditions_1
2D PDSD 700 ms	sample_2	solid	sample_conditions_1
2D NCA	sample_2	solid	sample_conditions_1
2D NCO	sample_2	solid	sample_conditions_1
2D NHHC	sample_2	solid	sample_conditions_1
2D PDSD 400 ms	sample_3	solid	sample_conditions_1
2D NCA	sample_3	solid	sample_conditions_1
2D NCO	sample_3	solid	sample_conditions_1
2D NHHC	sample_4	solid	sample_conditions_1
2D DQ-SQ SPC5	sample_1	solid	sample_conditions_1
2D NCACX DARR	sample_1	solid	sample_conditions_1
2D NCOCX DARR	sample_1	solid	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker Avance 850 MHz
Bruker Avance 800 MHz

BMRB	19346 19347 19348 19349
DBJ	BAB29375 BAE33670
GB	AAB20688 AAC00521 AAC16026 AAD11254 AAD56907
REF	NP_001035916 NP_033247 NP_062042 XP_003503776 XP_004648456
SP	O55042 P37377
AlphaFold	O55042 P37377

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
2	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
3	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
4	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
5	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
6	GLY	VAL	THR	THR	VAL	ALA	GLU	LYS	THR	LYS
7	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
8	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	ASN	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	MET
11	GLY	LYS	GLY	GLU	GLU	GLY	TYR	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	GLY	SER	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA

1	MET	ASP	VAL	PHE	MET	LYS	GLY	LEU	SER	LYS
2	ALA	LYS	GLU	GLY	VAL	VAL	ALA	ALA	ALA	GLU
3	LYS	THR	LYS	GLN	GLY	VAL	ALA	GLU	ALA	ALA
4	GLY	LYS	THR	LYS	GLU	GLY	VAL	LEU	TYR	VAL
5	GLY	SER	LYS	THR	LYS	GLU	GLY	VAL	VAL	HIS
6	GLY	VAL	THR	THR	VAL	ALA	GLU	LYS	THR	LYS
7	GLU	GLN	VAL	THR	ASN	VAL	GLY	GLY	ALA	VAL
8	VAL	THR	GLY	VAL	THR	ALA	VAL	ALA	GLN	LYS
9	THR	VAL	GLU	GLY	ALA	GLY	ASN	ILE	ALA	ALA
10	ALA	THR	GLY	PHE	VAL	LYS	LYS	ASP	GLN	MET
11	GLY	LYS	GLY	GLU	GLU	GLY	TYR	PRO	GLN	GLU
12	GLY	ILE	LEU	GLU	ASP	MET	PRO	VAL	ASP	PRO
13	GLY	SER	GLU	ALA	TYR	GLU	MET	PRO	SER	GLU
14	GLU	GLY	TYR	GLN	ASP	TYR	GLU	PRO	GLU	ALA