BMRB Entry 19348

Title:
mouse alpha synuclein N87S mutant- mouse/human chimera
Deposition date:
2013-07-09
Original release date:
2013-09-25
Authors:
Baum, Jean
Citation:

Citation: Kang, Lijuan; Janowska, Maria; Moriarty, Gina; Baum, Jean. "Mechanistic Insight into the Relationship between N-Terminal Acetylation of -Synuclein and Fibril Formation Rates by NMR and Fluorescence."  PLoS ONE 8, e75018-e75018 (2013).
PubMed: 24058647

Assembly members:

Assembly members:
aSyn_mouse_N87S, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: House Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p7-T7

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts128
1H chemical shifts128

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aSyn mouse_N87S monomer1

Entities:

Entity 1, aSyn mouse_N87S monomer 139 residues - Formula weight is not available

1   ASPVALPHEMETLYSGLYLEUSERLYSALA
2   LYSGLUGLYVALVALALAALAALAGLULYS
3   THRLYSGLNGLYVALALAGLUALAALAGLY
4   LYSTHRLYSGLUGLYVALLEUTYRVALGLY
5   SERLYSTHRLYSGLUGLYVALVALHISGLY
6   VALTHRTHRVALALAGLULYSTHRLYSGLU
7   GLNVALTHRASNVALGLYGLYALAVALVAL
8   THRGLYVALTHRALAVALALAGLNLYSTHR
9   VALGLUGLYALAGLYSERILEALAALAALA
10   THRGLYPHEVALLYSLYSASPGLNMETGLY
11   LYSGLYGLUGLUGLYTYRPROGLNGLUGLY
12   ILELEUGLUASPMETPROVALASPPROGLY
13   SERGLUALATYRGLUMETPROSERGLUGLU
14   GLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: aSyn_mouse_N87S, [U-100% 13C; U-100% 15N], 350 uM; H2O 90%; D2O 10%; Phosphate 10 mM; NaCl 138 mM; KCl 2 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 18232 19346 19347 19349
DBJ BAB29375 BAE33670
GB AAB20688 AAC00521 AAC16026 AAD11254 AAD56907
REF NP_001035916 NP_033247 NP_062042 XP_003503776 XP_005360611
SP O55042 P37377
AlphaFold O55042 P37377

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks