BMRB Entry 19348

Name: mouse alpha synuclein N87S mutant- mouse/human chimera
Published: 2013-09-25

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19348

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

mouse alpha synuclein N87S mutant- mouse/human chimera

Deposition date:

2013-07-09

Original release date:

2013-09-25

Authors:

Baum, Jean

Citation:

Citation: Kang, Lijuan; Janowska, Maria; Moriarty, Gina; Baum, Jean. "Mechanistic Insight into the Relationship between N-Terminal Acetylation of -Synuclein and Fibril Formation Rates by NMR and Fluorescence." PLoS ONE 8, e75018-e75018 (2013).
PubMed: 24058647

Assembly members:

Assembly members:
aSyn_mouse_N87S, polymer, 139 residues, Formula weight is not available

Natural source:

Natural source: Common Name: House Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: p7-T7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
aSyn_mouse_N87S: DVFMKGLSKAKEGVVAAAEK TKQGVAEAAGKTKEGVLYVG SKTKEGVVHGVTTVAEKTKE QVTNVGGAVVTGVTAVAQKT VEGAGSIAAATGFVKKDQMG KGEEGYPQEGILEDMPVDPG SEAYEMPSEEGYQDYEPEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	389
15N chemical shifts	128
1H chemical shifts	128

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	aSyn mouse_N87S monomer	1

Entities:

Entity 1, aSyn mouse_N87S monomer 139 residues - Formula weight is not available

1

ASP

VAL

PHE

MET

LYS

GLY

LEU

SER

LYS

ALA

2

LYS

GLU

GLY

VAL

ALA

GLU

LYS

3

THR

LYS

GLN

GLY

VAL

ALA

GLU

ALA

GLY

4

LYS

THR

LYS

GLU

GLY

VAL

LEU

TYR

VAL

GLY

5

SER

LYS

THR

LYS

GLU

GLY

VAL

HIS

GLY

6

VAL

THR

VAL

ALA

GLU

LYS

THR

LYS

GLU

7

GLN

VAL

THR

ASN

VAL

GLY

ALA

VAL

8

THR

GLY

VAL

THR

ALA

VAL

ALA

GLN

LYS

THR

9

VAL

GLU

GLY

ALA

GLY

SER

ILE

ALA

10

THR

GLY

PHE

VAL

LYS

ASP

GLN

MET

GLY

11

LYS

GLY

GLU

GLY

TYR

PRO

GLN

GLU

GLY

12

ILE

LEU

GLU

ASP

MET

PRO

VAL

ASP

PRO

GLY

13

SER

GLU

ALA

TYR

GLU

MET

PRO

SER

GLU

14

GLY

TYR

GLN

ASP

TYR

GLU

PRO

GLU

ALA

Samples:

sample_1: aSyn_mouse_N87S, [U-100% 13C; U-100% 15N], 350 uM; H2O 90%; D2O 10%; Phosphate 10 mM; NaCl 138 mM; KCl 2 mM

sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNN	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz

BMRB	18232 19346 19347 19349
DBJ	BAB29375 BAE33670
GB	AAB20688 AAC00521 AAC16026 AAD11254 AAD56907
REF	NP_001035916 NP_033247 NP_062042 XP_003503776 XP_005360611
SP	O55042 P37377
AlphaFold	O55042 P37377