BMRB Entry 18220
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PDB ID: 2lop
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18220
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Klammt, Christian; Maslennikov, Innokentiy; Bayrhuber, Monika; Eichmann, Cedric; Vajpai, Navratna; Chiu, Ellis Jeremy Chua; Blain, Katherine; Esquivies, Luis; Kwon, June Hyun Jung; Balana, Bartosz; Pieper, Ursula; Sali, Andrej; Slesinger, Paul; Kwiatkowski, Witek; Riek, Roland; Choe, Senyon. "Facile backbone structure determination of human membrane proteins by NMR spectroscopy." Nat. Methods 9, 834-839 (2012).
PubMed: 22609626
Assembly members:
entity, polymer, 108 residues, 10725.127 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: p23-GWN
Entity Sequences (FASTA):
entity: MTSLYKKVGMDLIGFGYAAL
VTFGSIFGYKRRGGVPSLIA
GLFVGCLAGYGAYRVSNDKR
DVKVSLFTAFFLATIMGVRF
KRSKKIMPAGLVAGLSLMMI
LRLVLLLL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 350 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 372 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TMEM14A | 1 |
Entities:
Entity 1, TMEM14A 108 residues - 10725.127 Da.
Residues 1-9 represent GW cloning tag.
| 1 | MET | THR | SER | LEU | TYR | LYS | LYS | VAL | GLY | MET | ||||
| 2 | ASP | LEU | ILE | GLY | PHE | GLY | TYR | ALA | ALA | LEU | ||||
| 3 | VAL | THR | PHE | GLY | SER | ILE | PHE | GLY | TYR | LYS | ||||
| 4 | ARG | ARG | GLY | GLY | VAL | PRO | SER | LEU | ILE | ALA | ||||
| 5 | GLY | LEU | PHE | VAL | GLY | CYS | LEU | ALA | GLY | TYR | ||||
| 6 | GLY | ALA | TYR | ARG | VAL | SER | ASN | ASP | LYS | ARG | ||||
| 7 | ASP | VAL | LYS | VAL | SER | LEU | PHE | THR | ALA | PHE | ||||
| 8 | PHE | LEU | ALA | THR | ILE | MET | GLY | VAL | ARG | PHE | ||||
| 9 | LYS | ARG | SER | LYS | LYS | ILE | MET | PRO | ALA | GLY | ||||
| 10 | LEU | VAL | ALA | GLY | LEU | SER | LEU | MET | MET | ILE | ||||
| 11 | LEU | ARG | LEU | VAL | LEU | LEU | LEU | LEU |
Related Database Links:
| BMRB | 18219 |
| PDB | 2LOO 2LOP |
| DBJ | BAG34999 |
| GB | AAD44496 AAF59948 AAH15097 AAH19328 ADQ32420 |
| REF | NP_001253566 NP_054770 XP_002746732 XP_002817046 XP_003254199 |
| SP | Q9Y6G1 |
| AlphaFold | Q9Y6G1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks