BMRB Entry 18047

Name: NMR structure of the protein NP_814968.1 from Enterococcus faecalis
Published: 2011-11-28

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PDB ID: 2llg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18047
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the protein NP_814968.1 from Enterococcus faecalis

Deposition date:

2011-11-08

Original release date:

2011-11-28

Authors:

Susac, Lukas; Serrano, Pedro; Geralt, Michael; Mohanty, Biswarajan; JCSG, JCSG; Wuthrich, Kurt

Citation:

Citation: Susac, Lukas; Serrano, Pedro; Geralt, Michael; Mohanty, Biswarajan; Wuthrich, Kurt. "NMR structure of the protein NP_814968.1 from Enterococcus faecalis" .

Assembly members:

Assembly members:
NP_814968.1, polymer, 143 residues, 16330.557 Da.

Natural source:

Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSpeedET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NP_814968.1: MDEVLATSIGTYENRTLLVP DGRLALTALHKGKDYQGKPM FYVLFELTNTTEKTQNIQLM IQSFMEVSQTVHGKAQNLQY AVLTDSPFQDKLDRLADEIN PGETIQGAYPYEFINENKPV HFKFRDRLLSLDEPIASEEI TIT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	484
15N chemical shifts	147
1H chemical shifts	986

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NP_814968.1	1

Entities:

Entity 1, NP_814968.1 143 residues - 16330.557 Da.

1

MET

ASP

GLU

VAL

LEU

ALA

THR

SER

ILE

GLY

2

THR

TYR

GLU

ASN

ARG

THR

LEU

VAL

PRO

3

ASP

GLY

ARG

LEU

ALA

LEU

THR

ALA

LEU

HIS

4

LYS

GLY

LYS

ASP

TYR

GLN

GLY

LYS

PRO

MET

5

PHE

TYR

VAL

LEU

PHE

GLU

LEU

THR

ASN

THR

6

THR

GLU

LYS

THR

GLN

ASN

ILE

GLN

LEU

MET

7

ILE

GLN

SER

PHE

MET

GLU

VAL

SER

GLN

THR

8

VAL

HIS

GLY

LYS

ALA

GLN

ASN

LEU

GLN

TYR

9

ALA

VAL

LEU

THR

ASP

SER

PRO

PHE

GLN

ASP

10

LYS

LEU

ASP

ARG

LEU

ALA

ASP

GLU

ILE

ASN

11

PRO

GLY

GLU

THR

ILE

GLN

GLY

ALA

TYR

PRO

12

TYR

GLU

PHE

ILE

ASN

GLU

ASN

LYS

PRO

VAL

13

HIS

PHE

LYS

PHE

ARG

ASP

ARG

LEU

SER

14

LEU

ASP

GLU

PRO

ILE

ALA

SER

GLU

ILE

15

THR

ILE

THR

Samples:

sample_1: NP_814968.1, [U-95% 13C; U-95% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.083 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
4D APSY HACANH	sample_1	isotropic	sample_conditions_1
5D APSY CBCACONH	sample_1	isotropic	sample_conditions_1
5D APSY HACACONH	sample_1	isotropic	sample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2LLG
EMBL	CBL31422 CCO72142 CPW23431 CRH64002
GB	AAO81038 ADX79912 AEA93699 AFO43932 AHI40381
REF	NP_814968 WP_002384665 WP_002395822 WP_002397838 WP_002419155