BMRB Entry 18029
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18029
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Citation: Banci, Lucia; Bertini, Ivano; Calderone, Vito; Cefaro, Chiara; Ciofi-Baffoni, Simone; Gallo, Angelo; Kallergi, Emmanouela; Lionaki, Eirini; Pozidis, Charalambos; Tokatlidis, Kostas. "Molecular recognition and substrate mimicry drive the electron-transfer process between MIA40 and ALR." Proc. Natl. Acad. Sci. U.S.A. 108, 4811-4816 (2011).
PubMed: 21383138
Assembly members:
sf-ALR, polymer, 129 residues, 14991.9 Da.
FAD, non-polymer, 785.550 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 24a(+)
Entity Sequences (FASTA):
sf-ALR: GSFTMRTQQKRDTKFREDCP
PDREELGRHSWAVLHTLAAY
YPDLPTPEQQQDMAQFIHLF
SKFYPCEECAEDLRKRLARN
HPDTRTRAAFTQWLCHLHNE
VNRKLGKPDFDCSKVDERWR
DGWKDGSCD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 411 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 503 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | sf-ALR, chain 1 | 1 |
| 2 | sf-ALR, chain 2 | 1 |
| 3 | FAD, 1 | 2 |
| 4 | FAD, 2 | 2 |
Entities:
Entity 1, sf-ALR, chain 1 129 residues - 14991.9 Da.
The first four residue (GSFT) are remaining from the TEV cleavage site
| 1 | GLY | SER | PHE | THR | MET | ARG | THR | GLN | GLN | LYS | ||||
| 2 | ARG | ASP | THR | LYS | PHE | ARG | GLU | ASP | CYS | PRO | ||||
| 3 | PRO | ASP | ARG | GLU | GLU | LEU | GLY | ARG | HIS | SER | ||||
| 4 | TRP | ALA | VAL | LEU | HIS | THR | LEU | ALA | ALA | TYR | ||||
| 5 | TYR | PRO | ASP | LEU | PRO | THR | PRO | GLU | GLN | GLN | ||||
| 6 | GLN | ASP | MET | ALA | GLN | PHE | ILE | HIS | LEU | PHE | ||||
| 7 | SER | LYS | PHE | TYR | PRO | CYS | GLU | GLU | CYS | ALA | ||||
| 8 | GLU | ASP | LEU | ARG | LYS | ARG | LEU | ALA | ARG | ASN | ||||
| 9 | HIS | PRO | ASP | THR | ARG | THR | ARG | ALA | ALA | PHE | ||||
| 10 | THR | GLN | TRP | LEU | CYS | HIS | LEU | HIS | ASN | GLU | ||||
| 11 | VAL | ASN | ARG | LYS | LEU | GLY | LYS | PRO | ASP | PHE | ||||
| 12 | ASP | CYS | SER | LYS | VAL | ASP | GLU | ARG | TRP | ARG | ||||
| 13 | ASP | GLY | TRP | LYS | ASP | GLY | SER | CYS | ASP |
Entity 2, FAD, 1 - C27 H33 N9 O15 P2 - 785.550 Da.
| 1 | FAD |
Related Database Links:
| BMRB | 26515 26533 |
| PDB | 3MBG 3O55 3TK0 3U2L 3U2M 4LDK |
| DBJ | BAI46852 |
| EMBL | CAB87993 |
| GB | AAA96390 AAD17328 AAD36986 AAG43494 AAH02429 |
| REF | NP_005253 XP_004057029 XP_007980253 |
| SP | P55789 |
| AlphaFold | P55789 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks