BMRB Entry 26515
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26515
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Citation: Kallergi, Emmanouela; Andreadaki, Maria; Kritsiligkou, Paraskevi; Katrakili, Nitsa; Pozidis, Charalambos; Tokatlidis, Kostas; Banci, Lucia; Bertini, Ivano; Cefaro, Chiara; Ciofi-Baffoni, Simone; Gajda, Karolina; Peruzzini, Riccardo. "Targeting and Maturation of Erv1/ALR in the Mitochondrial Intermembrane Space" ACS Chem. Biol. 7, 707-714 (2012).
PubMed: 22296668
Assembly members:
sf-ALR, polymer, 125 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSP64
Entity Sequences (FASTA):
sf-ALR: MRTQQKRDTKFREDCPPDRE
ELGRHSWAVLHTLAAYYPDL
PTPEQQQDMAQFIHLFSKFY
PCEECAEDLRKRLARNHPDT
RTRAAFTQWLCHLHNEVNRK
LGKPDFDCSKVDERWRDGWK
DGSCD
- heteronucl_NOEs
- heteronucl_T2_relaxation
| Data type | Count |
| heteronuclear NOE values | 43 |
| T2 relaxation values | 41 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FAD-free sf-ALR, chain 1 | 1 |
| 2 | FAD-free sf-ALR, chain 2 | 1 |
Entities:
Entity 1, FAD-free sf-ALR, chain 1 125 residues - Formula weight is not available
| 1 | MET | ARG | THR | GLN | GLN | LYS | ARG | ASP | THR | LYS | ||||
| 2 | PHE | ARG | GLU | ASP | CYS | PRO | PRO | ASP | ARG | GLU | ||||
| 3 | GLU | LEU | GLY | ARG | HIS | SER | TRP | ALA | VAL | LEU | ||||
| 4 | HIS | THR | LEU | ALA | ALA | TYR | TYR | PRO | ASP | LEU | ||||
| 5 | PRO | THR | PRO | GLU | GLN | GLN | GLN | ASP | MET | ALA | ||||
| 6 | GLN | PHE | ILE | HIS | LEU | PHE | SER | LYS | PHE | TYR | ||||
| 7 | PRO | CYS | GLU | GLU | CYS | ALA | GLU | ASP | LEU | ARG | ||||
| 8 | LYS | ARG | LEU | ALA | ARG | ASN | HIS | PRO | ASP | THR | ||||
| 9 | ARG | THR | ARG | ALA | ALA | PHE | THR | GLN | TRP | LEU | ||||
| 10 | CYS | HIS | LEU | HIS | ASN | GLU | VAL | ASN | ARG | LYS | ||||
| 11 | LEU | GLY | LYS | PRO | ASP | PHE | ASP | CYS | SER | LYS | ||||
| 12 | VAL | ASP | GLU | ARG | TRP | ARG | ASP | GLY | TRP | LYS | ||||
| 13 | ASP | GLY | SER | CYS | ASP |
Samples:
sample_1: FAD-free sf-ALR, [U-100% 15N], 0.5 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%
sample_conditions_1: temperature: 308 K; pH: 7.0; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN_1.3, Bruker Biospin - processing
CARA, Keller and Wuthrich - data analysis
CSI, Wishart, D. S., and Sykes, B. D. - chemical shift index analysis
PECAN, Wishart, D. S., and Sykes, B. D. - chemical shift index analysis
NMR spectrometers:
- Bruker NA 600 MHz