BMRB Entry 18015

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PDB ID: 2lkx
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18015
MolProbity Validation Chart

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Title:
Chemical shift assignments for the homeodomain of Pitx2 in complex with a TAATCC DNA binding site
Deposition date:
2011-10-21
Original release date:
2011-11-28
Authors:
Baird-Titus, Jamie; Doerdelmann, Thomas; Chaney, Beth; Clark-Baldwin, Kimber; Dave, Vrushank; Ma, Jun
Citation:

Citation: Chaney, Beth; Clark-Baldwin, Kimber; Dave, Vrushank; Ma, Jun; Rance, Mark. "Solution structure of the K50 class homeodomain PITX2 bound to DNA and implications for mutations that cause Rieger syndrome"  Biochemistry 44, 7497-7511 (2005).

Assembly members:

Assembly members:
PITX2, polymer, 68 residues, 8486.608 Da.
DNA_1, polymer, 11 residues, 3229.152 Da.
DNA_2, polymer, 11 residues, 3478.311 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-1lambdat-PITX2HD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PITX2: GSQRRQRTHFTSQQLQELEA TFQRNRYPDMSTREEIAVWT NLTEARVRVWFKNRRAKWRK REEFIVTD
DNA_1: CTCTAATCCCC
DNA_2: GGGGATTAGAG

Data sets:
Data typeCount
13C chemical shifts225
15N chemical shifts89
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PITX21
2DNA_12
3DNA_23

Entities:

Entity 1, PITX2 68 residues - 8486.608 Da.

Residues 61-66 represent a non-native cloning artifact. Residues -2, -1 represent are non-native, cloning artifact.

1   GLYSERGLNARGARGGLNARGTHRHISPHE
2   THRSERGLNGLNLEUGLNGLULEUGLUALA
3   THRPHEGLNARGASNARGTYRPROASPMET
4   SERTHRARGGLUGLUILEALAVALTRPTHR
5   ASNLEUTHRGLUALAARGVALARGVALTRP
6   PHELYSASNARGARGALALYSTRPARGLYS
7   ARGGLUGLUPHEILEVALTHRASP

Entity 2, DNA_1 11 residues - 3229.152 Da.

1   DCDTDCDTDADADTDCDCDC
2   DC

Entity 3, DNA_2 11 residues - 3478.311 Da.

1   DGDGDGDGDADTDTDADGDA
2   DG

Related Database Links:

BMRB 17145 17147 17452
PDB 2L7F 2L7M 2LKX
DBJ BAA75247 BAA75248 BAB69053 BAD12775 BAF84280
EMBL CAA06696 CAA06697 CAA09455 CAB52283 CAB52284
GB AAB38505 AAB38864 AAB87380 AAC16257 AAC24502
REF NP_000316 NP_001007500 NP_001017227 NP_001035436 NP_001035967
SP O35160 O75364 O93385 P81062 P97474
TPG DAA14908 DAA28868
AlphaFold O35160 O75364 O93385 P81062 P97474

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