BMRB Entry 17145

Title:
Chemical Shifts of the R24H Pitx2 Homeodomain Mutant
Deposition date:
2010-08-24
Original release date:
2010-11-09
Authors:
Doerdelmann, Thomas; Kojetin, Douglas; Baird-Titus, Jamie; Rance, Mark
Citation:

Citation: Doerdelmann, Thomas; Kojetin, Douglas; Baird-Titus, Jamie; Rance, Mark. "(1)H, (13)C and (15)N chemical shift assignments for the human Pitx2 homeodomain and a R24H homeodomain mutant."  Biomol. NMR Assignments 5, 105-107 (2011).
PubMed: 21052876

Assembly members:

Assembly members:
Pitx2_homeodomain_R24H, polymer, 68 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pitx2_homeodomain_R24H: GSQRRQRTHFTSQQLQELEA TFQRNHYPDMSTREEIAVWT NLTEARVRVWFKNRRAKWRK REEFIVTD

Data sets:
Data typeCount
13C chemical shifts310
15N chemical shifts78
1H chemical shifts466

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pitx2 homeodomain R24H1

Entities:

Entity 1, Pitx2 homeodomain R24H 68 residues - Formula weight is not available

1   GLYSERGLNARGARGGLNARGTHRHISPHE
2   THRSERGLNGLNLEUGLNGLULEUGLUALA
3   THRPHEGLNARGASNHISTYRPROASPMET
4   SERTHRARGGLUGLUILEALAVALTRPTHR
5   ASNLEUTHRGLUALAARGVALARGVALTRP
6   PHELYSASNARGARGALALYSTRPARGLYS
7   ARGGLUGLUPHEILEVALTHRASP

Samples:

sample_1: Pitx2 homeodomain R24H, [U-98% 13C; U-98% 15N], 1.5 mM; phosphate buffer 10 mM; sodium sulfate 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 17147 17452 18015
PDB
DBJ BAA75247 BAA75248 BAB69053 BAD12775 BAG36699
EMBL CAA06696 CAA06697 CAA09455 CAB52283 CAB52284
GB AAB38505 AAB38864 AAB87380 AAC24502 AAC27322
REF NP_000316 NP_001017227 NP_001035436 NP_001035967 NP_001035969
SP O35160 O75364 O93385 P81062 P97474
TPG DAA14908 DAA28868
AlphaFold P97474 O35160 O75364 O93385 P81062

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks