BMRB Entry 17899

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PDB ID: 2mu3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17899
MolProbity Validation Chart

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Title:
1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk
Deposition date:
2011-08-29
Original release date:
2011-10-25
Authors:
Xu, Lingling; Tremblay, Marie-Laurence; Meng, Qing; Liu, Xiang-Qin; Rainey, Jan
Citation:

Citation: Xu, Lingling; Tremblay, Marie-Laurence; Meng, Qing; Liu, Xiang-Qin; Rainey, Jan. "1H, 13C and 15N NMR assignments of the aciniform spidroin (AcSp1) repetitive domain of Argiope trifasciata wrapping silk."  Biomol. NMR Assignments 6, 147-151 (2012).
PubMed: 21989955

Assembly members:

Assembly members:
W1, polymer, 199 residues, 18976.5732 Da.

Natural source:

Natural source:   Common Name: Banded garden spider   Taxonomy ID: 156845   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Argiope Trifasciata

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
W1: AGPQGGFGATGGASAGLISR VANALANTSTLRTVLRTGVS QQIASSVVQRAAQSLASTLG VDGNNLARFAVQAVSRLPAG SDTSAYAQAFSSALFNAGVL NASNIDTLGSRVLSALLNGV SSAAQGLGINVDSGSVQSDI SSSSSFLSTSSSSASYSQAS ASSTSGAGYTGPSGPSTGPS GYPGPLGGGAPFGQSGFGG

Data typeCount
13C chemical shifts689
15N chemical shifts208
1H chemical shifts1092

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1W11

Entities:

Entity 1, W1 199 residues - 18976.5732 Da.

1   ALAGLYPROGLNGLYGLYPHEGLYALATHR
2   GLYGLYALASERALAGLYLEUILESERARG
3   VALALAASNALALEUALAASNTHRSERTHR
4   LEUARGTHRVALLEUARGTHRGLYVALSER
5   GLNGLNILEALASERSERVALVALGLNARG
6   ALAALAGLNSERLEUALASERTHRLEUGLY
7   VALASPGLYASNASNLEUALAARGPHEALA
8   VALGLNALAVALSERARGLEUPROALAGLY
9   SERASPTHRSERALATYRALAGLNALAPHE
10   SERSERALALEUPHEASNALAGLYVALLEU
11   ASNALASERASNILEASPTHRLEUGLYSER
12   ARGVALLEUSERALALEULEUASNGLYVAL
13   SERSERALAALAGLNGLYLEUGLYILEASN
14   VALASPSERGLYSERVALGLNSERASPILE
15   SERSERSERSERSERPHELEUSERTHRSER
16   SERSERSERALASERTYRSERGLNALASER
17   ALASERSERTHRSERGLYALAGLYTYRTHR
18   GLYPROSERGLYPROSERTHRGLYPROSER
19   GLYTYRPROGLYPROLEUGLYGLYGLYALA
20   PROPHEGLYGLNSERGLYPHEGLYGLY

Related Database Links:

BMRB TmpAcc 25197
PDB 2MU3
GB AAR83925

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