BMRB Entry 17720

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PDB ID: 2lep
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17720
MolProbity Validation Chart

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Title:
Solution Structure of N-terminal Cytosolic Domain of Rhomboid Intramembrane Protease from Escherichia Coli
Deposition date:
2011-06-20
Original release date:
2012-09-24
Authors:
Sherratt, Allison; Ghasriani, Houman; Goto, Natalie
Citation:

Citation: Sherratt, Allison; Blais, David; Ghasriani, Houman; Pezacki, John Paul; Goto, Natalie. "Activity-based protein profiling of the Escherichia coli GlpG rhomboid protein delineates the catalytic core."  Biochemistry 51, 7794-7803 (2012).
PubMed: 22963263

Assembly members:

Assembly members:
NGlpG, polymer, 63 residues, 7142.065 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET 30a +

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NGlpG: MLMITSFANPRVAQAFVDYM ATQGVILTIQQHNQSDVWLA DESQAERVRAELARFLENPA DLD

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts68
1H chemical shifts394

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1NGlpG1

Entities:

Entity 1, NGlpG 63 residues - 7142.065 Da.

1   METLEUMETILETHRSERPHEALAASNPRO
2   ARGVALALAGLNALAPHEVALASPTYRMET
3   ALATHRGLNGLYVALILELEUTHRILEGLN
4   GLNHISASNGLNSERASPVALTRPLEUALA
5   ASPGLUSERGLNALAGLUARGVALARGALA
6   GLULEUALAARGPHELEUGLUASNPROALA
7   ASPLEUASP

Related Database Links:

BMRB 19713
PDB 2LEP 2MJA
DBJ BAB37690 BAE77868 BAG79214 BAI27681 BAI32850
EMBL CAP77869 CAQ33744 CAR00366 CAR05025 CAR10074
GB AAG58528 AAN44906 AAN82639 AAP19275 AAT48182
REF NP_312294 NP_709199 WP_000541600 WP_000928708 WP_000928709
SP A1AGU7 A7ZSV4 A8A5N2 A8AQX4 B1IP42
AlphaFold A1AGU7 A7ZSV4 A8A5N2 A8AQX4 B1IP42

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