BMRB Entry 17693
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PDB ID: 2yka
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17693
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tunnicliffe, Richard; Hautbergue, G.; Kalra, P.; Jackson, B.; Whitehouse, A.; Wilson, S.; Golovanov, A.. "Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57" PLoS Pathog. 7, e1001244-e1001244 (2011).
PubMed: 21253573
Assembly members:
RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2, polymer, 124 residues, 13567.0128 Da.
52_KDA_IMMEDIATE-EARLY_PHOSPHOPROTEIN, polymer, 23 residues, 2504.8024 Da.
Natural source: Common Name: herpesvirus saimiri HVS Taxonomy ID: 10381 Superkingdom: Viruses Kingdom: not available Genus/species: Rhadinovirus Saimiriine herpesvirus 2
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b
Entity Sequences (FASTA):
RNA_AND_EXPORT_FACTOR-BINDING_PROTEIN_2: MASMTGGQQMGRDPDKWQHD
LFDSGCGGGEGVETGAKLLV
SNLDFGVSDADIQELFAEFG
TLKKAAVDYDRSGRSLGTAD
VHFERRADALKAMKQYKGVP
LDGRPMDIQLVASQIDLEHH
HHHH
52_KDA_IMMEDIATE-EARLY_PHOSPHOPROTEIN: GPLGSSCKTSWADRVREAAA
QRR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 535 |
| 15N chemical shifts | 153 |
| 1H chemical shifts | 922 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA AND EXPORT FACTOR-BINDING PROTEIN 2 | 1 |
| 2 | 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN | 2 |
Entities:
Entity 1, RNA AND EXPORT FACTOR-BINDING PROTEIN 2 124 residues - 13567.0128 Da.
| 1 | MET | ALA | SER | MET | THR | GLY | GLY | GLN | GLN | MET | ||||
| 2 | GLY | ARG | ASP | PRO | ASP | LYS | TRP | GLN | HIS | ASP | ||||
| 3 | LEU | PHE | ASP | SER | GLY | CYS | GLY | GLY | GLY | GLU | ||||
| 4 | GLY | VAL | GLU | THR | GLY | ALA | LYS | LEU | LEU | VAL | ||||
| 5 | SER | ASN | LEU | ASP | PHE | GLY | VAL | SER | ASP | ALA | ||||
| 6 | ASP | ILE | GLN | GLU | LEU | PHE | ALA | GLU | PHE | GLY | ||||
| 7 | THR | LEU | LYS | LYS | ALA | ALA | VAL | ASP | TYR | ASP | ||||
| 8 | ARG | SER | GLY | ARG | SER | LEU | GLY | THR | ALA | ASP | ||||
| 9 | VAL | HIS | PHE | GLU | ARG | ARG | ALA | ASP | ALA | LEU | ||||
| 10 | LYS | ALA | MET | LYS | GLN | TYR | LYS | GLY | VAL | PRO | ||||
| 11 | LEU | ASP | GLY | ARG | PRO | MET | ASP | ILE | GLN | LEU | ||||
| 12 | VAL | ALA | SER | GLN | ILE | ASP | LEU | GLU | HIS | HIS | ||||
| 13 | HIS | HIS | HIS | HIS |
Entity 2, 52 KDA IMMEDIATE-EARLY PHOSPHOPROTEIN 23 residues - 2504.8024 Da.
| 1 | GLY | PRO | LEU | GLY | SER | SER | CYS | LYS | THR | SER | ||||
| 2 | TRP | ALA | ASP | ARG | VAL | ARG | GLU | ALA | ALA | ALA | ||||
| 3 | GLN | ARG | ARG |
Related Database Links:
| UNP | REFP2_MOUSE IE63_SHV21 |
| BMRB | 16683 16697 16698 17663 17664 7435 |
| PDB | 2KT5 2YKA |
| EMBL | CAA45680 CAC84353 CAC84354 |
| GB | AAA46125 AAA66558 |
| PIR | WMBEHA |
| REF | NP_040259 |
| SP | P13199 |
| AlphaFold | Q8C869 P13199 P13199 |
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SPARKY: Backbone
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