BMRB Entry 7435

Name: HVS ORF57 8-120 backbone assignment, in complex with REF2-I 54-155.
Published: 2011-01-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7435

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

HVS ORF57 8-120 backbone assignment, in complex with REF2-I 54-155.

Deposition date:

2010-01-29

Original release date:

2011-01-25

Authors:

Tunnicliffe, Richard; Golovanov, Alexander; Wilson, Stuart; Hautbergue, Guillaume

Citation:

Citation: Tunnicliffe, Richard; Hautbergue, Guillaume; Kalra, Priti; Jackson, Brian; Whitehouse, Adrian; Wilson, Stuart; Golovanov, Alexander. "Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57." PLoS Pathog. 7, e1001244-. (2011).
PubMed: 21253573

Assembly members:

Assembly members:
REF_54-155, polymer, 124 residues, 13596.237 Da.
ORF57_8-120, polymer, 135 residues, 4268.970 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
REF_54-155: MASMTGGQQMGRDPDKWQHD LFDSGCGGGEGVETGAKLLV SNLDFGVSDADIQELFAEFG TLKKAAVDYDRSGRSLGTAD VHFERRADALKAMKQYKGVP LDGRPMDIQLVASQIDLEHH HHHH
ORF57_8-120: MASMTGGQQMGRDPGISSDD DFDSSDSSSDEEESDTSPQI MKSDVTMASPPSTPEPSLDV SASTSNLKRERQRSPITWEH QSPLSRVYRSPSPMRFGKRP RISSNSTSRSCKTSWADRVR EAAAQRRLEHHHHHH

Data sets:

assigned_chemical_shifts
- ORF57+REF_303k

Data type	Count
15N chemical shifts	104
1H chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	REF_54-155	1
2	ORF57_8-120	2

Entities:

Entity 1, REF_54-155 124 residues - 13596.237 Da.

Construct contains the following non-REF sequences: N-terminal T7 tag: MASMTGGQQMGRDP (assigned residue numbers 40-53) C-terminal His tag: LEHHHHHH (assigned residue numbers 156-163)

1

MET

ALA

SER

MET

THR

GLY

GLN

MET

2

GLY

ARG

ASP

PRO

ASP

LYS

TRP

GLN

HIS

ASP

3

LEU

PHE

ASP

SER

GLY

CYS

GLY

GLU

4

GLY

VAL

GLU

THR

GLY

ALA

LYS

LEU

VAL

5

SER

ASN

LEU

ASP

PHE

GLY

VAL

SER

ASP

ALA

6

ASP

ILE

GLN

GLU

LEU

PHE

ALA

GLU

PHE

GLY

7

THR

LEU

LYS

ALA

VAL

ASP

TYR

ASP

8

ARG

SER

GLY

ARG

SER

LEU

GLY

THR

ALA

ASP

9

VAL

HIS

PHE

GLU

ARG

ALA

ASP

ALA

LEU

10

LYS

ALA

MET

LYS

GLN

TYR

LYS

GLY

VAL

PRO

11

LEU

ASP

GLY

ARG

PRO

MET

ASP

ILE

GLN

LEU

12

VAL

ALA

SER

GLN

ILE

ASP

LEU

GLU

HIS

13

HIS

Entity 2, ORF57_8-120 135 residues - 4268.970 Da.

The construct contains the following non-ORF57 sequence at N-termini: MASMTGGQQMGRDP (assigned residue numbers -4 to 7) Plus the following at C-termini: LEHHHHHH (assigned residue numbers 121 to 128)

1

MET

ALA

SER

MET

THR

GLY

GLN

MET

2

GLY

ARG

ASP

PRO

GLY

ILE

SER

ASP

3

ASP

PHE

ASP

SER

ASP

SER

ASP

4

GLU

SER

ASP

THR

SER

PRO

GLN

ILE

5

MET

LYS

SER

ASP

VAL

THR

MET

ALA

SER

PRO

6

PRO

SER

THR

PRO

GLU

PRO

SER

LEU

ASP

VAL

7

SER

ALA

SER

THR

SER

ASN

LEU

LYS

ARG

GLU

8

ARG

GLN

ARG

SER

PRO

ILE

THR

TRP

GLU

HIS

9

GLN

SER

PRO

LEU

SER

ARG

VAL

TYR

ARG

SER

10

PRO

SER

PRO

MET

ARG

PHE

GLY

LYS

ARG

PRO

11

ARG

ILE

SER

ASN

SER

THR

SER

ARG

SER

12

CYS

LYS

THR

SER

TRP

ALA

ASP

ARG

VAL

ARG

13

GLU

ALA

GLN

ARG

LEU

GLU

HIS

14

HIS

Samples:

sample_1: ORF57 8-120, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; REF 54-155 2 ± 0.05 mM; phosphate buffer 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 288 K

sample_conditions_2: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_2
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_2

Software:

SPARKY v2.1, Goddard - data analysis

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance II+ 700 MHz

BMRB	16683 16697 17693 16698
PDB	2KT5 2YKA
EMBL	CAA45680 CAC84353 CAC84354
GB	AAA46125 AAA66558
PIR	WMBEHA
REF	NP_040259
SP	P13199
AlphaFold	P13199