BMRB Entry 17644

Name: Solution structure of the N-terminal domain of huntingtin (htt17) in presence of DPC micelles
Published: 2012-08-30

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PDB ID: 2ld2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17644
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal domain of huntingtin (htt17) in presence of DPC micelles

Deposition date:

2011-05-13

Original release date:

2012-08-30

Authors:

Michalek, Matthias; Salnikov, Evgeniy; Werten, Sebastiaan; Bechinger, Burkhard

Citation:

Citation: Michalek, Matthias; Salnikov, Evgeniy; Bechinger, Burkhard. "Structure and Topology of the Huntingtin 1-17 Membrane Anchor by a Combined Solution and Solid-State NMR Approach" Biophys. J. 105, 699-710 (2013).
PubMed: 23931318

Assembly members:

Assembly members:
htt17, polymer, 17 residues, 1977.422 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
htt17: MATLEKLMKAFESLKSF

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	72

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	htt17	1

Entities:

Entity 1, htt17 17 residues - 1977.422 Da.

1

MET

ALA

THR

LEU

GLU

LYS

LEU

MET

LYS

ALA

2

PHE

GLU

SER

LEU

LYS

SER

PHE

Samples:

sample_1: htt17, 15N labeled at PHE11, 0.2-0.3 mM; D2O 10%; sodium phosphate 25 mM; DPC, d38, 100 mM; H2O 90%

sample_conditions_1: ionic strength: 0.025 M; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

X-PLOR NIH, Bhattacharya and Montelione, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Koradi, Billeter and Wuthrich, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

Bruker Avance 600 MHz

BMRB	17642 25146
PDB	2LD0 2LD2 3IO4 3IO6 3IOR 3IOT 3IOU 3IOV 3IOW 4FE8 4FEB 4FEC 4FED 4RAV
DBJ	BAA36752 BAA36753 BAH03657
EMBL	CAA12281
GB	AAA89100 AAB38240 AAC52218 AAI72756 AAR34461
REF	NP_001136110 NP_001254674 NP_002102 NP_034544 NP_077333
SP	P42858 P42859
TPG	DAA28444
AlphaFold	P42858 P42859