BMRB Entry 25146

Name: Solid-state NMR chemical shifts of amyloid-like fibrils formed by huntingtin N-terminal fragments (httNTQ30P10K2)
Published: 2014-10-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25146

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR chemical shifts of amyloid-like fibrils formed by huntingtin N-terminal fragments (httNTQ30P10K2)

Deposition date:

2014-08-12

Original release date:

2014-10-14

Authors:

Hoop, Cody; Kar, Karunakar; Wetzel, Ronald; van der Wel, Patrick

Citation:

Citation: Hoop, Cody; Lin, Hsiang-Kai; Kar, Karunakar; Hou, Zhipeng; Poirier, Michelle; Wetzel, Ronald; van der Wel, Patrick. "Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR" Biochemistry 53, 6653-6666 (2014).
PubMed: 25280367

Assembly members:

Assembly members:
httNTQ30P10K2, polymer, 59 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
httNTQ30P10K2: MATLEKLMKAFESLKSFQQQ QQQQQQQQQQQQQQQQQQQQ QQQQQQQPPPPPPPPPPKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_chainA
- assigned_chem_shift_list_chainB

Data type	Count
13C chemical shifts	138
15N chemical shifts	12

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	httNTQ30P10K2, Chain A	1
2	httNTQ30P10K2, Chain B	1

Entities:

Entity 1, httNTQ30P10K2, Chain A 59 residues - Formula weight is not available

1

MET

ALA

THR

LEU

GLU

LYS

LEU

MET

LYS

ALA

2

PHE

GLU

SER

LEU

LYS

SER

PHE

GLN

3

GLN

4

GLN

5

GLN

PRO

6

PRO

LYS

Samples:

LQP-labeled: httNTQ30P10K2, [U-13C; U-15N]- L7, Q47, P48, 8 mg

LAQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- L4, A10, Q46, 8 mg

LKSQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- L4, K6, S16, Q19, 1.8 mg

MA-labeled: httNTQ30P10K2, [U-13C; U-15N]- M8, A10, 8 mg

ALF-labeled: httNTQ30P10K2, [U-13C; U-15N]- A2, L7, F17, 2 mg

AFLQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- A10, F11, L14, Q18, 3 mg

275_K: temperature: 275 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR	LQP-labeled	solid	275_K
2D 13C-13C DARR	LAQ-labeled	solid	275_K
2D 13C-13C DARR	LKSQ-labeled	solid	275_K
2D 13C-13C DARR	MA-labeled	solid	275_K
2D 15N-13C TEDOR	LQP-labeled	solid	275_K
2D 13C-13C DARR	ALF-labeled	solid	275_K
2D 13C-13C DARR	AFLQ-labeled	solid	275_K

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz