BMRB Entry 17414

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PDB ID: 2l8l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17414
MolProbity Validation Chart

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Title:
Structure of an engineered splicing intein mutant based on Mycobacterium tuberculosis RecA
Deposition date:
2011-01-19
Original release date:
2012-01-04
Authors:
Du, Zhenming; Wang, Chunyu
Citation:

Citation: Du, Zhenming; Zheng, Yuchuan; Liu, Yangzhong; Belfort, Marlene; Wang, Chunyu. "pK(a) coupling at the intein active site: implications for the coordination mechanism of protein splicing with a conserved aspartate"  J. Am. Chem. Soc. 133, 10275-10282 (2011).
PubMed: 21604815

Assembly members:

Assembly members:
RecA, polymer, 139 residues, 15365.621 Da.

Natural source:

Natural source:   Common Name: high GC Gram+   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM41

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RecA: CLAEGTRIFDPVTGTTHRIE DVVGGRKPIHVVAAAKDGTL HARPVVSWFDQGTRDVIGLR IAGGAILWATPDHKVLTEYG WRAAGELRKGDRVAVRDVET GELRYSVIREVLPTRRARTF DLEVEELHTLVAEGVVVHN

Data sets:
Data typeCount
13C chemical shifts288
15N chemical shifts137
1H chemical shifts904

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RecA1

Entities:

Entity 1, RecA 139 residues - 15365.621 Da.

1   CYSLEUALAGLUGLYTHRARGILEPHEASP
2   PROVALTHRGLYTHRTHRHISARGILEGLU
3   ASPVALVALGLYGLYARGLYSPROILEHIS
4   VALVALALAALAALALYSASPGLYTHRLEU
5   HISALAARGPROVALVALSERTRPPHEASP
6   GLNGLYTHRARGASPVALILEGLYLEUARG
7   ILEALAGLYGLYALAILELEUTRPALATHR
8   PROASPHISLYSVALLEUTHRGLUTYRGLY
9   TRPARGALAALAGLYGLULEUARGLYSGLY
10   ASPARGVALALAVALARGASPVALGLUTHR
11   GLYGLULEUARGTYRSERVALILEARGGLU
12   VALLEUPROTHRARGARGALAARGTHRPHE
13   ASPLEUGLUVALGLUGLULEUHISTHRLEU
14   VALALAGLUGLYVALVALVALHISASN

Related Database Links:

BMRB 15560 16634
PDB 2IN0 2IN8 2IN9 2L8L 3IFJ 3IGD
EMBL CFR77613 CMM98736
GB EPZ65824 KAM73204
REF WP_049963343

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