BMRB Entry 15560

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15560

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Title:
1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA
Deposition date:
2007-11-19
Original release date:
2008-06-05
Authors:
Du, Zhenming; Liu, Yangzhong; Zheng, Yuchuan; Dansereau, John; Belfort, Marlene; Belfort, Georges; Van Roey, Patrick; Wang, Chunyu
Citation:

Citation: Du, Zhenming; Liu, Yangzhong; Zheng, Yuchuan; McCallum, Scott; Dansereau, John; Derbyshire, Victoria; Belfort, Marlene; Belfort, Georges; Van Roey, Patrick; Wang, Chunyu. "1H, 13C, and 15N NMR assignments of an engineered intein based on Mycobacterium tuberculosis RecA"  Biomol. NMR Assignments 2, 111-113 (2008).
PubMed: 19636882

Assembly members:

Assembly members:
DDI-CM, polymer, 139 residues, 15282.4 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BME21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DDI-CM: CLAEGTRIFDPVTGTTHRIE DVVGGRKPIHVVAAAKDGTL HARPVVSWFDQGTRDVIGLR IAGGAILWATPDHKVLTEYG WRAAGELRKGDRVAVRDVET GELRYSVIREVLPTRRARTF GLEVEELHTLVAEGVVVHN

Data sets:
Data typeCount
13C chemical shifts596
15N chemical shifts145
1H chemical shifts965

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sununit 11

Entities:

Entity 1, sununit 1 139 residues - 15282.4 Da.

1   CYSLEUALAGLUGLYTHRARGILEPHEASP
2   PROVALTHRGLYTHRTHRHISARGILEGLU
3   ASPVALVALGLYGLYARGLYSPROILEHIS
4   VALVALALAALAALALYSASPGLYTHRLEU
5   HISALAARGPROVALVALSERTRPPHEASP
6   GLNGLYTHRARGASPVALILEGLYLEUARG
7   ILEALAGLYGLYALAILELEUTRPALATHR
8   PROASPHISLYSVALLEUTHRGLUTYRGLY
9   TRPARGALAALAGLYGLULEUARGLYSGLY
10   ASPARGVALALAVALARGASPVALGLUTHR
11   GLYGLULEUARGTYRSERVALILEARGGLU
12   VALLEUPROTHRARGARGALAARGTHRPHE
13   GLYLEUGLUVALGLUGLULEUHISTHRLEU
14   VALALAGLUGLYVALVALVALHISASN

Samples:

CM_mutant_H2O_sample: DDI-CM, [U-98% 13C; U-98% 15N], 0.3 ± 0.05 mM; sodium phosphate 50 ± 1 mM; sodium azide 1 ± 0.1 mM; sodium chloride 100 ± 5 mM; H2O 10%; D2O 90%

CM_mutant_D2O_sample: DDI-CM, [U-98% 13C; U-98% 15N], 0.3 ± 0.05 mM; sodium phosphate 50 ± 5 mM; sodium azide 1 ± 0.1 mM; sodium chloride 100 ± 5 mM; D2O 90%

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACM_mutant_H2O_sampleisotropicsample_conditions_1
3D HNCACBCM_mutant_H2O_sampleisotropicsample_conditions_1
3D HNCOCM_mutant_H2O_sampleisotropicsample_conditions_1
3D CBCA(CO)NHCM_mutant_H2O_sampleisotropicsample_conditions_1
3D 1H-15N NOESYCM_mutant_H2O_sampleisotropicsample_conditions_1
3D 1H-15N TOCSYCM_mutant_H2O_sampleisotropicsample_conditions_1
3D H(CCO)NHCM_mutant_H2O_sampleisotropicsample_conditions_1
3D C(CO)NHCM_mutant_H2O_sampleisotropicsample_conditions_1
3D 1H-13C NOESYCM_mutant_D2O_sampleisotropicsample_conditions_1
3D HCCH-TOCSYCM_mutant_D2O_sampleisotropicsample_conditions_1
2D 1H-15N HSQCCM_mutant_H2O_sampleisotropicsample_conditions_1
2D 1H-13C HSQCCM_mutant_D2O_sampleisotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment, peak picking, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16634 17414
PDB
EMBL CFR77613 CMM98736
GB EPZ65824 KAM73204
REF WP_049963343

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks