BMRB Entry 17407

Name: The complex structure of homeodomain in solution
Published: 2012-06-04

Click here to enlarge.

PDB ID: 2lp0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17407
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

The complex structure of homeodomain in solution

Deposition date:

2011-01-12

Original release date:

2012-06-04

Authors:

LIU, Changdong; ZHOU, Bo; ZHU, Guang

Citation:

Citation: Zhou, Bo; Liu, Changdong; Xu, Zhiwen; Zhu, Guang. "Structural basis for homeodomain recognition by the cell-cycle regulator Geminin." Proc. Natl. Acad. Sci. U.S.A. 109, 8931-8936 (2012).
PubMed: 22615398

Assembly members:

Assembly members:
entity_1, polymer, 60 residues, 7739.270 Da.
entity_2, polymer, 20 residues, 2330.259 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet32

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TRKKRCPYTKYQTLELEKEF LFNMYLTRDRRYEVARVLNL TERQVKIWFQNRRMKMKKMN
entity_2: NQEFDSEEETVEDSLVEDSE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	300
15N chemical shifts	88
1H chemical shifts	550

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	entity_2	2

Entities:

Entity 1, entity_1 60 residues - 7739.270 Da.

1	THR	ARG	LYS	LYS	ARG	CYS	PRO	TYR	THR	LYS
2	TYR	GLN	THR	LEU	GLU	LEU	GLU	LYS	GLU	PHE
3	LEU	PHE	ASN	MET	TYR	LEU	THR	ARG	ASP	ARG
4	ARG	TYR	GLU	VAL	ALA	ARG	VAL	LEU	ASN	LEU
5	THR	GLU	ARG	GLN	VAL	LYS	ILE	TRP	PHE	GLN
6	ASN	ARG	ARG	MET	LYS	MET	LYS	LYS	MET	ASN

Entity 2, entity_2 20 residues - 2330.259 Da.

1		ASN	GLN	GLU	PHE	ASP	SER	GLU	GLU	GLU	THR
2		VAL	GLU	ASP	SER	LEU	VAL	GLU	ASP	SER	GLU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 13C-filtered 13C-edited NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCACB	sample_3	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_3	isotropic	sample_conditions_1

Related Database Links:

BMRB	25142
PDB	2LP0 2MSY 2LP0 2WVR
DBJ	BAA86253 BAB28077 BAC37378 BAE44282 BAE53490 BAD96712 BAG51040 BAI46867
EMBL	CAA30487 CAA39026 CAJ82440 CBL59361 CDQ56636
GB	AAA37856 AAB04756 AAB68680 AAD15960 AAF44633 AAC39787 AAH05185 AAH05389 ADQ32794 AIC51377
PIR	C45187 D45187
REF	NP_001017256 NP_001094377 NP_001133005 NP_001133012 NP_001133018 NP_001238918 NP_001238919 NP_001238920 NP_056979 XP_001171903
SP	O42502 P09633 P31274 Q28601 Q9IA13 O75496
TPG	DAA30041
AlphaFold	O42502 P09633 P31274 Q28601 Q9IA13 O75496

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 17407

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	THR	ARG	LYS	LYS	ARG	CYS	PRO	TYR	THR	LYS
2	TYR	GLN	THR	LEU	GLU	LEU	GLU	LYS	GLU	PHE
3	LEU	PHE	ASN	MET	TYR	LEU	THR	ARG	ASP	ARG
4	ARG	TYR	GLU	VAL	ALA	ARG	VAL	LEU	ASN	LEU
5	THR	GLU	ARG	GLN	VAL	LYS	ILE	TRP	PHE	GLN
6	ASN	ARG	ARG	MET	LYS	MET	LYS	LYS	MET	ASN

1		ASN	GLN	GLU	PHE	ASP	SER	GLU	GLU	GLU	THR
2		VAL	GLU	ASP	SER	LEU	VAL	GLU	ASP	SER	GLU

1	THR	ARG	LYS	LYS	ARG	CYS	PRO	TYR	THR	LYS
2	TYR	GLN	THR	LEU	GLU	LEU	GLU	LYS	GLU	PHE
3	LEU	PHE	ASN	MET	TYR	LEU	THR	ARG	ASP	ARG
4	ARG	TYR	GLU	VAL	ALA	ARG	VAL	LEU	ASN	LEU
5	THR	GLU	ARG	GLN	VAL	LYS	ILE	TRP	PHE	GLN
6	ASN	ARG	ARG	MET	LYS	MET	LYS	LYS	MET	ASN

1		ASN	GLN	GLU	PHE	ASP	SER	GLU	GLU	GLU	THR
2		VAL	GLU	ASP	SER	LEU	VAL	GLU	ASP	SER	GLU

1	THR	ARG	LYS	LYS	ARG	CYS	PRO	TYR	THR	LYS
2	TYR	GLN	THR	LEU	GLU	LEU	GLU	LYS	GLU	PHE
3	LEU	PHE	ASN	MET	TYR	LEU	THR	ARG	ASP	ARG
4	ARG	TYR	GLU	VAL	ALA	ARG	VAL	LEU	ASN	LEU
5	THR	GLU	ARG	GLN	VAL	LYS	ILE	TRP	PHE	GLN
6	ASN	ARG	ARG	MET	LYS	MET	LYS	LYS	MET	ASN

1		ASN	GLN	GLU	PHE	ASP	SER	GLU	GLU	GLU	THR
2		VAL	GLU	ASP	SER	LEU	VAL	GLU	ASP	SER	GLU