BMRB Entry 17034

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17034

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Title:
1H and 15N Chemical Shift Assignments for the Syrian hamster prion protein shPrP(90-232)
Deposition date:
2010-06-30
Original release date:
2011-01-19
Authors:
Bjorndahl, Trent; Zhou, Guo-Ping; Lui, Xue-Hui; Perez-Pineiro, Rolando; Semenchenko, Valentyna; Bujold, Adina; Sobsey, Constance; Wishart, David
Citation:

Citation: Bjorndahl, Trent; Zhou, Guo-Ping; Liu, Xuehui; Perez-Pineiro, Rolando; Semenchenko, Valentyna; Saleem, Fozia; Acharya, Sandipta; Bujold, Adina; Sobsey, Constance; Wishart, David. "Detailed Biophysical Characterization of the Acid-Induced PrP(c) to PrP() Conversion Process."  Biochemistry 50, 1162-1173 (2011).
PubMed: 21189021

Assembly members:

Assembly members:
shPrP, polymer, 162 residues, 18710.7 Da.

Natural source:

Natural source:   Common Name: Golden hamster   Taxonomy ID: 10036   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesocricetus auratus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
shPrP: MGSSHHHHHHSSGLVPRGSG QGGGTHNQWNKPSKPKTNMK HMAGAAAAGAVVGGLGGYML GSAMSRPMMHFGNDWEDRYY RENMNRYPNQVYYRPVDQYN NQNNFVHDCVNITIKQHTVT TTTKGENFTETDIKIMERVV EQMCTTQYQKESQAYYDGRR SS

Data typeCount
15N chemical shifts544
1H chemical shifts1791

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1shPrP(90-232)1

Entities:

Entity 1, shPrP(90-232) 162 residues - 18710.7 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERGLY
3   GLNGLYGLYGLYTHRHISASNGLNTRPASN
4   LYSPROSERLYSPROLYSTHRASNMETLYS
5   HISMETALAGLYALAALAALAALAGLYALA
6   VALVALGLYGLYLEUGLYGLYTYRMETLEU
7   GLYSERALAMETSERARGPROMETMETHIS
8   PHEGLYASNASPTRPGLUASPARGTYRTYR
9   ARGGLUASNMETASNARGTYRPROASNGLN
10   VALTYRTYRARGPROVALASPGLNTYRASN
11   ASNGLNASNASNPHEVALHISASPCYSVAL
12   ASNILETHRILELYSGLNHISTHRVALTHR
13   THRTHRTHRLYSGLYGLUASNPHETHRGLU
14   THRASPILELYSILEMETGLUARGVALVAL
15   GLUGLNMETCYSTHRTHRGLNTYRGLNLYS
16   GLUSERGLNALATYRTYRASPGLYARGARG
17   SERSER

Related Database Links:

BMRB 17834 4307
PDB 1B10 2LH8 4YXL
GB AAA37090 AAA37091 AAA37092 AAA37093 AAB25731
REF XP_005068717 XP_012967855
SP P04273
AlphaFold P04273

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