BMRB Entry 4307

Name: 1H, 13C, and 15N Chemical Shift Assignments for SHa rPrP(90-231).
Published: 2000-05-10

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4307

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for SHa rPrP(90-231).

Deposition date:

1999-02-16

Original release date:

2000-05-10

Authors:

James, Thomas; Liu, He; Ulyanov, Nikolai; Far-Jones, Shauna

Citation:

Citation: Liu, H.; Farr-Jones, S.; Ulyanov, N.; Llinas, M.; Marqusee, S.; Groth, D.; Cohen, F.; Prusiner, S.; James, T.. "Solution structure of Syrian hamster prion protein rPrP(90-231)" Biochemistry 38, 5362-5377 (1999).
PubMed: 10220323

Assembly members:

Assembly members:
recombinant Syrian hamster prion protein, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Syrian hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Microcricetus aureus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
recombinant Syrian hamster prion protein: GQGGGTHNQWNKPSKPKTNM KHMAGAAAAGAVVGGLGGYM LGSAMSRPMMHFGNDWEDRY YRENMNRYPNQVYYRPVDQY NNQNNFVHDCVNITIKQHTV TTTTKGENFTETDIKIMERV VEQMCTTQYQKESQAYYDGR RS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	401
15N chemical shifts	168
1H chemical shifts	862

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	rPrP(90-231)	1

Entities:

Entity 1, rPrP(90-231) 142 residues - Formula weight is not available

1

GLY

GLN

GLY

THR

HIS

ASN

GLN

TRP

2

ASN

LYS

PRO

SER

LYS

PRO

LYS

THR

ASN

MET

3

LYS

HIS

MET

ALA

GLY

ALA

GLY

4

ALA

VAL

GLY

LEU

GLY

TYR

MET

5

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

MET

6

HIS

PHE

GLY

ASN

ASP

TRP

GLU

ASP

ARG

TYR

7

TYR

ARG

GLU

ASN

MET

ASN

ARG

TYR

PRO

ASN

8

GLN

VAL

TYR

ARG

PRO

VAL

ASP

GLN

TYR

9

ASN

GLN

ASN

PHE

VAL

HIS

ASP

CYS

10

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

THR

VAL

11

THR

LYS

GLY

GLU

ASN

PHE

THR

12

GLU

THR

ASP

ILE

LYS

ILE

MET

GLU

ARG

VAL

13

VAL

GLU

GLN

MET

CYS

THR

GLN

TYR

GLN

14

LYS

GLU

SER

GLN

ALA

TYR

ASP

GLY

ARG

15

ARG

SER

Samples:

sample_1: recombinant Syrian hamster prion protein, [U-13C; U-15N], 0.7 – 1.3 mM

cond._set_1: pH: 5.2; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCO	not available	not available	not available
HBHA(CO)NH	not available	not available	not available
C(CO)NH-TOCSY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
1H-15N NOESY-HSQC	not available	not available	not available

Software:

SPARKY v3.6 - anotation, assignment, bookkeeping, peak picking

NMR spectrometers:

Bruker AMX 500 MHz
Bruker DRX 600 MHz
Bruker RMX 750 MHz
Varian Unity Plus 600 MHz

BMRB	17034 17834
PDB	1B10 2LH8 4YXL
GB	AAA37090 AAA37091 AAA37092 AAA37093 AAB25731
REF	XP_005068717 XP_012967855
SP	P04273
AlphaFold	P04273